NFIA 抗体
Quick Overview for NFIA 抗体 (ABIN7126744)
抗原
See all NFIA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, i±-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
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交叉反应 （详细）
- Human, Mouse and Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment of human NFIA protein corresponding to protein domain
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亚型
- IgG2
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anti-Nuclear Factor I/A (NFIA) (AA 1-498) antibody
NFIA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E11 unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-330) antibodyNFIA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-224) antibodyNFIA 适用: 人, 小鼠, 大鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-224), (Middle Region) antibodyNFIA 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-498) antibodyNFIA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 348-397) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 豚鼠, Pig, 小鸡, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 384-418), (C-Term) antibodyNFIA 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB51284 unconjugated
anti-Nuclear Factor I/A (NFIA) (C-Term) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-143) antibodyNFIA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (Middle Region) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 犬, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Human fetal gut, colon, liver or heart.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- NFIA (Nuclear Factor I/A (NFIA))
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别名
- NFIA
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背景
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CCAAT-box-binding transcription factor, CTF, NF I/A, NF-I/A, NF1-A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1 A-type, TGGCA binding protein,NFIA / NF1A (Nuclear Factor 1A) (Transcription Factor)
Cellular localisation: Nucleus. -
分子量
- 55kDa
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基因ID
- 4774, 322431
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UniProt
- Q15784
抗原
-
