Recombinant MLH1 抗体
Quick Overview for Recombinant MLH1 抗体 (ABIN7126704)
抗原
See all MLH1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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特异性
- This MAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human MLH1 protein
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亚型
- IgG
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anti-MutL Homolog 1 (MLH1) (AA 1-756) antibody
MLH1 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal M1 unconjugated
anti-MutL Homolog 1 (MLH1) (AA 381-483) antibodyMLH1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7A7C8 unconjugated
anti-MutL Homolog 1 (MLH1) (AA 381-483) antibodyMLH1 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 8C12B7 unconjugated
anti-MutL Homolog 1 (MLH1) (AA 350-519) antibodyMLH1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) (AA 381-483) antibodyMLH1 适用: 人, 猴 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 4C9C7 unconjugated
anti-MutL Homolog 1 (MLH1) (Internal Region) antibodyMLH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) antibodyMLH1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) (AA 501-600) antibodyMLH1 适用: 人 ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) (Internal Region) antibodyMLH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-MutL Homolog 1 (MLH1) (AA 373-735) antibodyMLH1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human tonsil or colon carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MLH1 (MutL Homolog 1 (MLH1))
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别名
- MLH1
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背景
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COCA2, DNA mismatch repair protein, FCC2, hMLH1, MutL homolog 1 colon cancer nonpolyposis type 2 (HNPCC2),MLH1 (MutL Homolog 1) / HNPCC
Cellular localisation: Nuclear -
分子量
- 85kDa
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基因ID
- 4292, 195364
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UniProt
- P40692
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
-
