MEF2D 抗体
Quick Overview for MEF2D 抗体 (ABIN7126680)
抗原
See all MEF2D 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5 and MRF4, are one class of identified factors. A second family of DNA-binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.
-
交叉反应 （详细）
- Human. Predicted to react in Mouse and Rat.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant full-length human MEF2D protein
-
亚型
- IgG2b
-
-
anti-Myocyte Enhancer Factor 2D (MEF2D) (AA 299-509) antibody
MEF2D 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myocyte Enhancer Factor 2D (MEF2D) (AA 256-351) antibodyMEF2D 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-Myocyte Enhancer Factor 2D (MEF2D) (AA 1-521) antibodyMEF2D 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Myocyte Enhancer Factor 2D (MEF2D) (AA 1-110) antibodyMEF2D 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myocyte Enhancer Factor 2D (MEF2D) (AA 1-521) antibodyMEF2D 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Myocyte Enhancer Factor 2D (MEF2D) (AA 256-351) antibodyMEF2D 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-Myocyte Enhancer Factor 2D (MEF2D) (AA 256-351) antibodyMEF2D 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D6 unconjugated
anti-Myocyte Enhancer Factor 2D (MEF2D) (pSer180) antibodyMEF2D 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Myocyte Enhancer Factor 2D (MEF2D) (pSer444) antibodyMEF2D 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- MEF2D (Myocyte Enhancer Factor 2D (MEF2D))
-
别名
- MEF2D
-
背景
-
MADS box transcription factor 2 polypeptide D, Myocyte specific enhancer factor 2, polypeptide D,MEF2D / Myocyte enhancer factor 2D (Transcription Factor)
Cellular localisation: Nucleus. Translocated by HDAC4 to nuclear dots. -
分子量
- 55.94kDa
-
基因ID
- 4209, 314327
-
UniProt
- Q14814
抗原
-
