MBP 抗体
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- 抗原 See all MBP products
- MBP
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适用
- 大鼠, 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This MBP antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Coating (Coat)
- 特异性
- Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4275 is used in clinical diagnostics to detect MBP levels or myelination in human MBP. MBP/4275 is used in a two-site ELISA with clone MBP/4273. Both are used interchangeably as capture and detection antibody.
- 交叉反应 （详细）
- Human, Chicken, Guinea Pig, Rat, Pig, Rabbit, Sheep and Mammals.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Spleen cells from an immunised outbred rat were fused with cells of the mouse NS0 myeloma cell line.
- 亚型
- IgG2a
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anti-MBP (AA 69-85) antibody
适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MBP (AA 1-197) antibody适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-MBP (pThr232) antibody适用: 人, 大鼠, 小鼠 ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MBP antibody适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 10C12 unconjugated
anti-MBP antibody适用: 人 WB, IF 宿主: 小鼠 Monoclonal 408CT17-4-3 unconjugated
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- 应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Immunocytochemistry (0.5-1 μg/mL), ,Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human brain or astrocytoma tissue.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- MBP
- Abstract
- MBP 产品
- 背景
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GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP,Myelin Basic Protein
Cellular localisation: Myelin membrane, Peripheral membrane protein, Cytoplasmic side (of myelin). - 分子量
- 13-21kDa
- 基因ID
- 4155, 551713
- UniProt
- P02686
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