MBP 抗体
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北京 101111
Quick Overview for MBP 抗体 (ABIN7126669)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Myelin Basic Protein Antibody
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特异性
- Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4271 recognizes an epitope in the 129-138 region of MBP, useful in clinical diagnosis to detect MBP levels.
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交叉反应 （详细）
- Human, bovine and rat.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Reacts with MBP from human, bovine and rat, recognizing epitope 129-138
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亚型
- IgG2a
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anti-MBP (AA 69-85) antibody
适用: 豚鼠 WB, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MBP (AA 1-197) antibody适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-MBP (C-Term) antibody适用: 人, 大鼠, 小鼠, Pig WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-MBP antibody适用: 人 WB, IF 宿主: 小鼠 Monoclonal 408CT17-4-3 unconjugated
anti-MBP (pThr232) antibody适用: 人 FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MBP (pTyr203) antibody适用: 人 DB 宿主: 兔 Polyclonal RB15524 unconjugated
anti-MBP (AA 177-209) antibody适用: 人 WB 宿主: 兔 Polyclonal RB15523 unconjugated
anti-MBP antibody适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 10C12 unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Immunocytochemistry (0.5-1 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human brain or astrocytoma tissue.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- MBP
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背景
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GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP
Human brain or astrocytoma tissue. Mouse Brain, Rat Brain or Hamster Brain
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分子量
- 14-22kDa
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基因ID
- 4155, 551713
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UniProt
- P02686
抗原
-
