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Recombinant MLANA 抗体

This 小鼠 单克隆 anti-MLANA antibody specifically detects MLANA in IHC (f). The antibody is reactive with 人 samples.
产品编号 ABIN7126407
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for Recombinant MLANA 抗体 (ABIN7126407)

抗原

See all MLANA 抗体
MLANA (Melan A (MLANA))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MLANA antibody is un-conjugated

应用范围

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Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
  • 特异性

    This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant full-length human MART-1 protein

    亚型

    IgG2a
  • 应用备注

    Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: SK-MEL-13 or SK-MEL-19 melanoma cell lines. Melanomas.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    MLANA (Melan A (MLANA))

    别名

    MLANA

    背景

    Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA,MART-1 / Melan-A / MLANA (Melanoma Marker)
    Cellular localisation: Cytoplasmic

    分子量

    20-22kDa (doublet)

    基因ID

    2315, 154069

    UniProt

    Q16655
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