EIF4A2 抗体
Quick Overview for EIF4A2 抗体 (ABIN7126324)
抗原
See all EIF4A2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5'-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5' cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.
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交叉反应 （详细）
- Human. Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human EIF4A2 protein
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亚型
- IgG2a
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anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-270) antibody
EIF4A2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-407) antibodyEIF4A2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 333-360), (C-Term) antibodyEIF4A2 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB23766 unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibodyEIF4A2 适用: 人 WB, IHC (p), IF, ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 5-31), (N-Term) antibodyEIF4A2 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibodyEIF4A2 适用: 人, 大鼠, 小鼠 WB, IHC, IF, IP, RIP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 339-369), (C-Term) antibodyEIF4A2 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) antibodyEIF4A2 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-100) antibodyEIF4A2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4A2 (EIF4A2) (AA 1-50) antibodyEIF4A2 适用: 人, 大鼠, 小鼠, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order Ab without BSA),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))
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别名
- EIF4A2
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背景
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ATP dependent RNA helicase eIF4A 2, BM-010, DDX2B, EIF4F, eukaryotic translation initiation factor 4A isoform 2, N-terminally processed,EIF4A2 (Eukaryotic Initiation Factor 4A-II)
Cellular localisation: Nucleus. -
分子量
- 46kDa
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基因ID
- 1974
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UniProt
- Q14240
抗原
-
