E4F1 抗体
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北京 101111
Quick Overview for E4F1 抗体 (ABIN7126308)
抗原
See all E4F1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities.
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交叉反应 （详细）
- Human
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human E4F1 protein
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亚型
- IgG1
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anti-E4F Transcription Factor 1 (E4F1) (AA 685-784) antibody
E4F1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-E4F Transcription Factor 1 (E4F1) (AA 1-784) antibodyE4F1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-E4F Transcription Factor 1 (E4F1) (AA 685-784) antibodyE4F1 适用: 人 WB, IF, ELISA, IHC (p), IHC 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-E4F Transcription Factor 1 (E4F1) (AA 1-784) antibodyE4F1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, Jurkat or MCF7 cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- E4F1 (E4F Transcription Factor 1 (E4F1))
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别名
- E4F1
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背景
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E4F transcription factor 1, isoform CRA_b, P120E4F transcription factor, p50E4F, Putative E3 ubiquitin-protein ligase E4F1, RING type E3 ubiquitin transferase E4F1, Transcription factor E4F1,E4F1
Cellular localisation: Nucleus. -
分子量
- 83kDa
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基因ID
- 1877, 513268
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UniProt
- Q66K89
抗原
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