E4F1 抗体
Quick Overview for E4F1 抗体 (ABIN7126308)
抗原
See all E4F1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities.
-
交叉反应 （详细）
- Human
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant full-length human E4F1 protein
-
亚型
- IgG1
-
-
anti-E4F Transcription Factor 1 (E4F1) (AA 685-784) antibody
E4F1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-E4F Transcription Factor 1 (E4F1) (AA 1-784) antibodyE4F1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-E4F Transcription Factor 1 (E4F1) (AA 1-784) antibodyE4F1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, Jurkat or MCF7 cells.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- E4F1 (E4F Transcription Factor 1 (E4F1))
-
别名
- E4F1
-
背景
-
E4F transcription factor 1, isoform CRA_b, P120E4F transcription factor, p50E4F, Putative E3 ubiquitin-protein ligase E4F1, RING type E3 ubiquitin transferase E4F1, Transcription factor E4F1,E4F1
Cellular localisation: Nucleus. -
分子量
- 83kDa
-
基因ID
- 1877, 513268
-
UniProt
- Q66K89
抗原
-
