DCP2 抗体
Quick Overview for DCP2 抗体 (ABIN7126292)
抗原
See all DCP2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3' exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay
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交叉反应 （详细）
- Human. Predicted to react with Mouse and Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human DCP2 protein
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亚型
- IgG2a
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anti-DCP2 Decapping Enzyme Homolog (DCP2) (AA 1-240) antibody
DCP2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DCP2 Decapping Enzyme Homolog (DCP2) (AA 1-385) antibodyDCP2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DCP2 Decapping Enzyme Homolog (DCP2) (C-Term) antibodyDCP2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DCP2 Decapping Enzyme Homolog (DCP2) (AA 1-385) antibodyDCP2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-DCP2 Decapping Enzyme Homolog (DCP2) (AA 144-173) antibodyDCP2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23240 unconjugated
anti-DCP2 Decapping Enzyme Homolog (DCP2) (AA 151-181), (Middle Region) antibodyDCP2 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-DCP2 Decapping Enzyme Homolog (DCP2) (AA 265-314) antibodyDCP2 适用: 人, 小鼠, 大鼠, 豚鼠, 马, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-DCP2 Decapping Enzyme Homolog (DCP2) (AA 324-373) antibodyDCP2 适用: 人, 豚鼠, 犬, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), ,Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: MCF7, Daudi or Jurkat cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))
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别名
- DCP2
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背景
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DCP2 decapping enzyme homolog (S. cerevisiae), DFLJ33245, hDpc, m7GpppN-mRNA hydrolase, mRNA decapping enzyme 2, nudix (nucleoside diphosphate linked moiety X)-type motif 20 (NUDT20),DCP2 (Decapping mRNA 2)
Cellular localisation: Cytoplasm > P. body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear. -
分子量
- 48.4kDa
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基因ID
- 167227, 443875
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UniProt
- Q8IU60
抗原
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