Recombinant NeuN 抗体 (AA 30-60)
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- 抗原 See all NeuN (RBFOX3) 抗体
- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
- 抗体类型
- Recombinant Antibody
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抗原表位
- AA 30-60
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This NeuN antibody is un-conjugated
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应用范围
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function, however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- A synthetic peptide corresponding to residues within aa 30-60 of human NeuN protein (exact sequence is proprietary)
- 亚型
- IgG
- Top Product
- Discover our top product RBFOX3 Primary Antibody
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anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody
RBFOX3 适用: 人, 小鼠 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1H3B2 unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-220) antibodyRBFOX3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibodyRBFOX3 适用: 人, 小鼠 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5A4B9 unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 51-150) antibodyRBFOX3 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyRBFOX3 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyRBFOX3 适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyRBFOX3 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyRBFOX3 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Brain tissue.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
- 别名
- RBFOX3 (RBFOX3 产品)
- 别名
- FOX-3 antibody, FOX3 antibody, HRNBP3 antibody, NEUN antibody, Fox-3 antibody, Hrnbp3 antibody, NeuN antibody, Neuna60 antibody, Neun antibody, RGD1560070 antibody, RNA binding fox-1 homolog 3 antibody, RNA binding protein, fox-1 homolog (C. elegans) 3 antibody, RNA binding protein, fox-1 homolog 3 antibody, RBFOX3 antibody, Rbfox3 antibody
- 背景
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FLJ56884, FLJ58356, fox1 homolog C, Fox3, FOX3NeuN, hexaribonucleotide binding protein 3 (HRNBP3), neuronal nuclei, Rbfox3, RNA binding protein, fox 1 homolog (C. elegans) 3,Neuronal-Nuclei (NeuN) (Neuronal Marker)
Cellular localisation: Nucleus. Cytoplasm. - 分子量
- 38kDa
- 基因ID
- 146713, 135229
- UniProt
- A6NFN3
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