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Recombinant OLIG2 抗体 (AA 200-300)

This anti-OLIG2 antibody is a 兔 单克隆 antibody detecting OLIG2 in IHC (f). Suitable for 人.
产品编号 ABIN7504157
发货至: 中国

Quick Overview for Recombinant OLIG2 抗体 (AA 200-300) (ABIN7504157)

抗原

See all OLIG2 抗体
OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This OLIG2 antibody is un-conjugated

应用范围

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Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
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    AA 200-300

    特异性

    Olig2, a basic helix loop helix transcription factor, is involved in oligo-dendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuroepithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid/rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue.

    交叉反应 (详细)

    Human.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant fragment (around aa200-300) of human OLIG2 protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    Positive Control: THP-1 cells. Human brain or astrocytoma.

    Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

    有效期

    24 months
  • 抗原

    OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

    别名

    OLIG2

    背景

    Basic helix loop helix protein class B1 (bHLHB1), basic helix-loop-helix protein 19 (bHLHe19), OLIG2, Oligodendrocyte lineage transcription factor 2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, Protein kinase C-binding protein 2 (PRKCBP2), RACK17,OLIG2 (Marker of Glial Brain Tumors)
    Cellular localisation: Nucleus. Cytoplasm.

    分子量

    32kDa

    基因ID

    10215, 176977

    UniProt

    Q13516
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