Vimentin 抗体
Quick Overview for Vimentin 抗体 (ABIN7120771)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- VIM antibody
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纯化方法
- Protein A+G purification
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纯度
- ≥95 % as determined by SDS-PAGE
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免疫原
- vimentin
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亚型
- IgG1
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应用备注
- WB: 1:1000-1:10000, IHC: 1:200-1:2000, IF: 1:20-1:200
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze / thaw cycles.
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储存条件
- -20 °C
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储存方法
- -20°C for 12 months
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有效期
- 12 months
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- Vimentin (VIM)
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别名
- VIM
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背景
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Synonyms: Vimentin|VIM
Background: Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA, 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
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分子量
- 55 kDa
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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