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Vimentin 抗体

This 小鼠 单克隆 antibody specifically detects Vimentin in WB, IHC 和 ELISA. It exhibits reactivity toward 人, 大鼠 和 小鼠.
产品编号 ABIN7120771
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Vimentin 抗体 (ABIN7120771)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, 大鼠, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This Vimentin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

克隆位点

2G10
  • 原理

    VIM antibody

    纯化方法

    Protein A+G purification

    纯度

    ≥95 % as determined by SDS-PAGE

    免疫原

    vimentin

    亚型

    IgG1
  • 应用备注

    WB: 1:1000-1:10000, IHC: 1:200-1:2000, IF: 1:20-1:200

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze / thaw cycles.

    储存条件

    -20 °C

    储存方法

    -20°C for 12 months

    有效期

    12 months
  • 抗原

    Vimentin (VIM)

    别名

    VIM

    背景

    Synonyms: Vimentin|VIM

    Background: Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA, 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

    分子量

    55 kDa

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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