MSH6 抗体
Quick Overview for MSH6 抗体 (ABIN7116545)
抗原
See all MSH6 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A+G purification
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纯度
- ≥95 % as determined by SDS-PAGE
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免疫原
- mutS homolog 6
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亚型
- IgG2a
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anti-MutS Homolog 6 (E. Coli) (MSH6) antibody
MSH6 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3E1 unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 374-540) antibodyMSH6 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 7D9H5 unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 374-540) antibodyMSH6 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 2E3A10 unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 931-1030) antibodyMSH6 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 1F2 unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 374-540) antibodyMSH6 适用: 人 WB, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal MSH6-3091 unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 217-395) antibodyMSH6 适用: 人, 小鼠, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 1D1C2 unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) (N-Term) antibodyMSH6 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, Cow, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) (Internal Region) antibodyMSH6 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) antibodyMSH6 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5B11 unconjugated
anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 1-400) antibodyMSH6 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:50-1:200
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
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有效期
- 12 months
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- MSH6 (MutS Homolog 6 (E. Coli) (MSH6))
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别名
- MSH6
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背景
- Synonyms:G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160 Background:Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3(H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
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分子量
- 160 kDa
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基因ID
- 2956
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UniProt
- P52701
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途径
- DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response
抗原
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