MGEA5 抗体
Quick Overview for MGEA5 抗体 (ABIN7116207)
抗原
See all MGEA5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- OGA antibody
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纯化方法
- Protein A+G purification
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纯度
- ≥95 % as determined by SDS-PAGE
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免疫原
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Immunogen sequence: 1-400
Immunogen: meningioma expressed antigen 5(hyaluronidase)
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亚型
- IgG2a
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应用备注
- WB: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze / thaw cycles.
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储存条件
- -20 °C
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储存方法
- -20°C for 12 months
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有效期
- 12 months
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- MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))
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别名
- OGA
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背景
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Synonyms: Protein O-GlcNAcase (OGA)|Beta-N-acetylglucosaminidase|Beta-N-acetylhexosaminidase|Beta-hexosaminidase|Meningioma-expressed antigen 5|N-acetyl-beta-D-glucosaminidase|N-acetyl-beta-glucosaminidase|Nuclear cytoplasmic O-GlcNAcase and acetyltransferase (NCOAT)|OGA|HEXC|KIAA0679|MEA5|MGEA5
Background: Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro)(PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity(PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro), but has about six times lower specific activity than isoform 1.
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分子量
- 130 kDa
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基因ID
- 10724
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UniProt
- O60502
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途径
- Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process
抗原
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