LIN28B 抗体
Quick Overview for LIN28B 抗体 (ABIN7115882)
抗原
See all LIN28B 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- LIN28B antibody
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纯化方法
- Immunogen affinity purified
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纯度
- ≥95 % as determined by SDS-PAGE
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免疫原
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Immunogen sequence: 5-250AA
Immunogen: lin-28 homolog B
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亚型
- IgG
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anti-Lin-28 Homolog B (LIN28B) (AA 1-30) antibody
LIN28B 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 95-128) antibodyLIN28B 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 166-250) antibodyLIN28B 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) antibodyLIN28B 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 218-250) antibodyLIN28B 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 95-128) antibodyLIN28B 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) antibodyLIN28B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 200-250) antibodyLIN28B 适用: 人, 猴 IHC, IHC (p), IP 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (N-Term) antibodyLIN28B 适用: 人 WB, ELISA, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (Center) antibodyLIN28B 适用: 人 WB, FACS, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500-1:2000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze / thaw cycles.
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储存条件
- -20 °C
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储存方法
- -20°C for 12 months
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有效期
- 12 months
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- LIN28B (Lin-28 Homolog B (LIN28B))
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别名
- LIN28B
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背景
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Synonyms: Protein lin-28 homolog B (Lin-28B)|LIN28B|CSDD2
Background: Suppressor of microRNA(miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts(pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA(PubMed:22118463). Does not act on pri-miR21(PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation(PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent(PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression(By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
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分子量
- 27 kDa
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基因ID
- 389421
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UniProt
- Q6ZN17
抗原
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