DNA2 抗体
Quick Overview for DNA2 抗体 (ABIN7113558)
抗原
See all DNA2 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- DNA2 antibody
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纯化方法
- Immunogen affinity purified
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纯度
- ≥95 % as determined by SDS-PAGE
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免疫原
- DNA replication helicase 2 homolog
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亚型
- IgG
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anti-DNA Replication Helicase 2 Homolog (DNA2) (AA 1-300) antibody
DNA2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA Replication Helicase 2 Homolog (DNA2) antibodyDNA2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA Replication Helicase 2 Homolog (DNA2) (Internal Region) antibodyDNA2 适用: 人, 小鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500-1:2000, IHC: 1:50-1:500
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze / thaw cycles.
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储存条件
- -20 °C
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储存方法
- -20°C for 12 months
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有效期
- 12 months
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- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
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别名
- DNA2
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背景
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Synonyms: DNA replication ATP-dependent helicase/nuclease DNA2 (hDNA2)|DNA replication ATP-dependent helicase-like homolog|DNA2|DNA2L|KIAA0083
Background: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
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分子量
- 127 kDa
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基因ID
- 1763
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UniProt
- P51530
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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