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BrdU 抗体

The 小鼠 单克隆 anti-BrdU antibody (Clone 9E8) (ABIN7112073) specifically detects BrdU in IHC, IF 和 ELISA. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7112073
发货至: 中国
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Quick Overview for BrdU 抗体 (ABIN7112073)

抗原

See all BrdU 抗体
BrdU (Bromodeoxyuridine (BrdU))

适用

  • 4
  • 1
  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 45
  • 25
  • 8
  • 2
小鼠

克隆类型

  • 55
  • 25
单克隆

标记

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  • 2
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  • 2
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This BrdU antibody is un-conjugated

应用范围

  • 27
  • 27
  • 23
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  • 20
  • 13
  • 11
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  • 7
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Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

克隆位点

9E8
  • 原理

    BrdU antibody

    纯化方法

    Protein A+G purification

    纯度

    ≥95 % as determined by SDS-PAGE

    免疫原

    Immunogen sequence: compound

    Immunogen: compound

    亚型

    IgG2a
  • 应用备注

    IHC: 1:200-1:1000, IF: 1:50-1:500

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze / thaw cycles.

    储存条件

    -20 °C

    储存方法

    -20°C for 12 months

    有效期

    12 months
  • 抗原

    BrdU (Bromodeoxyuridine (BrdU))

    别名

    BrdU

    物质类

    Chemical

    背景

    Synonyms: 5-bromo-2-deoxyuridine|BrDu|BrdUrd|Bromodeoxyuridine|BUdR

    Background: Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

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