Recombinant Transferrin Receptor 抗体
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Quick Overview for Recombinant Transferrin Receptor 抗体 (ABIN7072542)
抗原
See all Transferrin Receptor (TFRC) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Transferrin receptor [RI7 217.1.3], Rabbit IgG, kappa
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特异性
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The transferrin receptor is necessary for cellular iron uptake by the process of receptor-mediated endocytosis. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis.
Application Notes original Clone: This antibody was generated and used in a flow cytometry assay to identify murine hematopoietic progenitor cells expressing the transferrin receptor (Lesley et al., 1984, PMID: 6319033). The original format of this antibody was tested and found to moderately reduce cell growth. A Fab version of this antibody was generated, its binding was tested, and it was compared to its full antibody counterpart. In contrast to the original antibody format, the Fab fragment did not cause receptor down-regulation or accelerated degradation. Both the Fab fragment and the full antibody were successfully used in an IF staining assay (Lesley et al., 1989, PMID: 2653853). The original fotmat of this antibody was demonstrated to undergo receptor-mediated transcytosis across the blood-brain barrier (BBB), as confirmed by capillary depletion studies, and to effectively transport into the mouse brain in vivo (Lee et al., 2000, PMID: 10688622). Injection of this antibody into mice resulted in CD71+ erythroid splenocyte cell depletion (Torow et al., 2015, PMID: 26195040).
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产品特性
- This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
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纯化方法
- Protein A affinity purified
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免疫原
- The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.
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亚型
- IgG, kappa
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anti-Transferrin Receptor (p90, CD71) (TFRC) antibody
TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibodyTFRC 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 578-765) antibodyTFRC 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 1-140) antibodyTFRC 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 1-198) antibodyTFRC 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) antibodyTFRC 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 608-727) antibodyTFRC 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 小鼠 Monoclonal 1A1B2 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibody (Biotin)TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 Biotin
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 649-677), (C-Term) antibodyTFRC 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB20736 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 621-720) antibodyTFRC 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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OriginalSpeciesName: Rat
OriginalFormat: IgG2a
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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别名
- Transferrin receptor
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背景
- CD71, Transferrin receptor protein 1, TR, , TfR1, Trfr, R17 217.1.3, RI7-217
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途径
- Transition Metal Ion Homeostasis
抗原
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