Recombinant CD35 抗体
Quick Overview for Recombinant CD35 抗体 (ABIN7072539)
抗原
See all CD35 (CR1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CR1 [E11], Rabbit IgG, kappa
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特异性
- E11 is reactive to neutrophilic granulocytes and eosinophils, monocytes, macrophages, lymphocytes, erythrocytes, dendritic reticulum cells, tonsil tissue section, spleen tissue section, lymph node tissue section, gut tissue section, kidney podocytes and kidney tissue sections, chronic lymphocytic leukemia, acute myelomonocytic leukemia, acute monocyte leukemia, B cell lines (JY, MST, BR17), EBV-infected B cell cultures, myeloid cell lines (U937, HL60, THP 1-0).
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- BALB/c mice were immunised with cells from acute monocytic leukemia patients.
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亚型
- IgG kappa
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应用备注
- E11 fluorescence staining of normal blood cells was detectable on 90 % of monocytes, 95 % of neutrophils, and 14 %-24 % of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
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别名
- CR1
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背景
- CD35, Type 1 complement receptor, C3b/C4b receptor
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途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
抗原
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