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Recombinant CD35 抗体

This anti-CD35 antibody is a 小鼠 单克隆 antibody detecting CD35 in WB, IF 和 ELISA. Suitable for 人.
产品编号 ABIN7072538
发货至: 中国

Quick Overview for Recombinant CD35 抗体 (ABIN7072538)

抗原

See all CD35 (CR1) 抗体
CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD35 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), ELISA

克隆位点

E11
  • 原理

    Anti-CR1 [E11], Mouse IgG1, kappa

    特异性

    E11 is reactive to neutrophilic granulocytes and eosinophils, monocytes, macrophages, lymphocytes, erythrocytes, dendritic reticulum cells, tonsil tissue section, spleen tissue section, lymph node tissue section, gut tissue section, kidney podocytes and kidney tissue sections, chronic lymphocytic leukemia, acute myelomonocytic leukemia, acute monocyte leukemia, B cell lines (JY, MST, BR17), EBV-infected B cell cultures, myeloid cell lines (U937, HL60, THP 1-0).

    产品特性

    Original Species of Ab: Mouse

    Original Format of Ab: IgG1

    纯化方法

    Protein A affinity purified

    免疫原

    BALB/c mice were immunised with cells from acute monocytic leukemia patients.

    亚型

    IgG1 kappa
  • 应用备注

    E11 fluorescence staining of normal blood cells was detectable on 90 % of monocytes, 95 % of neutrophils, and 14 %-24 % of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013).

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

    别名

    CR1

    背景

    CD35, Type 1 complement receptor, C3b/C4b receptor

    途径

    Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
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