Recombinant Galectin 9 抗体
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Quick Overview for Recombinant Galectin 9 抗体 (ABIN7072508)
抗原
See all Galectin 9 (LGALS9) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Galectin 9 [RG9-35], Rat IgG2a, kappa
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特异性
- This antibody is specific for murine galectin-9, a 40 kDa S-type lectin that is expressed by various cell types, including lymphocytes, thymocytes, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, epithelial cells, and endothelial cells. Galectin-9 binds to β-galactosides and can serve as a ligand for TIM-3 (CD366). The protein is implicated in both innate and adaptive immune responses, specifically, induction of cytokine secretion by macrophages, bactericidal functions, promotion of dendritic cell maturation, regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells.
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by immunising rats with recombinant mouse galectin-9.
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亚型
- IgG2a kappa
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anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 225-353) antibody
LGALS9 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-323) antibodyLGALS9 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 51-150) antibodyLGALS9 适用: 人, 小鼠 WB, ELISA, FACS, IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 101-180) antibodyLGALS9 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-240) antibodyLGALS9 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 254-355) antibodyLGALS9 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (Internal Region) antibodyLGALS9 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 194-322) antibodyLGALS9 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (Center) antibodyLGALS9 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (Internal Region) antibodyLGALS9 适用: 人, 小鼠, 大鼠 WB, IP, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- When this antibody was first generated and characterised, it was shown to bind with galectin-9 (Gal-9) but not with either galectin-1 or galectin-3. Furthermore, it was shown to be capable of blocking Tim-3-Fc binding to Gal-9, and inhibiting Gal-9-induced Th1 cell death in vitro (Fukushima et al., 2008, PMID: 18087160). This antibody has been used in FACS analyses. For instance, to describe a novel mechanism where Tim-3 binds HMGB-1 on virus-specific CD8+ Treg cells, suppressing proliferation of CD8+ T effector cells during acute adenoviral infection (Dolina et al., 2014, PMID: 24677194), and to indicate a crucial role for Tim-3 in phagocytosis of apoptotic cells and cross-presentation (Nakayama et al., 2009, PMID: 19224762). In addition, this antibody has been used in vivo to study the roles of Gal-9 in the development of experimental allergic conjunctivitis in mice (Fukushima et al., 2008, PMID: 18087160) and to demonstrate that constitutive expression of Gal-9 plays an immunosuppressive role in corneal allografts (Shimmura-Tomita et al., 2013, PMID: 23667648). This antibody was used in a cold immunoprecipitation (IP) assay to explore the binding of Gal-Nab1 and Gal-Nab2 to native murine gal-9, using a protein extract from murine thymus as the source of native Gal-9, RG9-35, was one of the antibodies used to isolate immune complexes formed between primary antibodies and proteins extracted from a mouse thymus for further analysis (Lhuillier et al., 2018, PMID: 30204750).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))
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别名
- Galectin 9
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背景
- Ecalectin, Gal-9, galectin 9, Galectin-9, HUAT, lectin, galactose binding, soluble 5, LGALS9A, tumor antigen HOM-HD-21, urate transporter/channel protein.
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UniProt
- O08573
抗原
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