Recombinant GM2 Ganglioside 抗体
This 兔 Chimeric antibody specifically detects GM2 Ganglioside in ELISA, IHC, Dep 和 TLC Overlay. It exhibits reactivity toward 人.
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产品编号 ABIN7072396
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for Recombinant GM2 Ganglioside 抗体 (ABIN7072396)
抗原
GM2 Ganglioside
抗体类型
Recombinant Antibody
适用
人
宿主
兔
克隆类型
Chimeric
标记
非结合性
应用范围
ELISA, Immunohistochemistry (IHC), Depletion (Dep), Thin Layer Chromatography Overlay (TLC Overlay)
克隆位点
L55-81
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原理
- Anti-GM2 ganglioside [L55-81], Rabbit IgG, kappa
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特异性
- L55-81 binds specifically to GM2 ganglioside, this antibody shows no reactivity towards GM1, GM3, GD2 or normal tissues- the L55-81 epitope contains both a GalNAc and sialic acid bound to lactose. Ganglioside GM2 (GalNAcβ1-4(NeuNAcα2-3)Galβ1-4Glcβ-1ceramide) is a glycosphingolipid present on the cell surface and a major tumor-differentiation antigen in human melanoma and hepatoma.
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产品特性
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Original Species of Ab: Human
Original Format of Ab: IgM
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纯化方法
- Protein A affinity purified
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免疫原
- L55-81 was prepared by isolating EBV-immortalized peripheral blood B lymphocytes from a human donor with melanoma.
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亚型
- IgG kappa
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应用备注
- L55-81 reacts positively to melanoma cell lines with high GM2 expression (M14, M24), and shows no binding to normal cell types or cell lines M15 or M25 which have low GM2 expression and high GM3 expression. This specificity can be confirmed by ELISA and TLC immunostaining by testing a variety of gangliosides and neutral glycolipids. L55-81 is able to lyse cells from the M14 melanoma cell line (which has high GM2 expression) in the presence of human or rabbit complement, but not in the absence of complement - this was shown by a [51Cr] release assay. Biotinylated L55-81 has been used for IHC of acetone-fixed sections of tumor tissue in an avidin-biotin complex immunoperoxidase system. Statistically significant positive staining (determined by Fisher's exact test) was displayed with melanoma, colon carcinoma and ovary carcinoma tumor tissues. Staining has also be observed with breast carcinoma, prostate carcinoma and kidney carcinoma.
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- GM2 Ganglioside
抗原
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