VAMP2 抗体 (Cytosolic, N-Term)
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Quick Overview for VAMP2 抗体 (Cytosolic, N-Term) (ABIN7043878)
抗原
See all VAMP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-27, Cytosolic, N-Term
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原理
- A Rabbit Polyclonal Antibody to VAMP-2
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交叉反应
- 小鼠, 大鼠
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预测反应
- Human,bovine,Macaca mulata - 25,26 amino acid residues identical
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产品特性
- Anti-v Antibody is directed against the intracellular N-terminal domain of rat V. Anti-V Antibody (ABIN7043878, ABIN7044653 and ABIN7044654) can be used in western blot and immunohistochemical applications, and has been designed to recognize V from rat, mouse and human samples.
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纯化方法
- The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
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免疫原
- GST fusion protein with the sequence SATAATVPPAAPAGEGGPPAPPPNLT, corresponding to amino acid residues 2-27 of rat VAMP2
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亚型
- IgG
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anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-100) antibody
VAMP2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibodyVAMP2 适用: 人, 大鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3-00E-005 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) antibodyKD Validated VAMP2 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB1855 unconjugated Recombinant Antibody
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (N-Term) antibodyVAMP2 适用: 人, 小鼠, 大鼠, Hamster WB, IHC, ICC, IP, IHC (p) 宿主: 豚鼠 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) antibodyVAMP2 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (N-Term) antibodyVAMP2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-50) antibodyVAMP2 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:1000
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7566008)
Blocking Peptide: (ABIN7566008)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))
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别名
- VAMP2
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背景
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Synonyms: Vesicle-associated membrane protein 2, Synaptobrevin 2, Syb2
Description: VAMP-2 (also known as synaptobrevin-2) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionary conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.VAMP-2, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located on the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.VAMP-2 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as the vesicle membrane protein v-SNARE, which together with the plama membrane t-SNARE protein Syntaxin 1 and the membrane-associated SNAP-25 (synaptosome-associated protein 25 kDa), forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.VAMP-2 is the target of the tetanus neurotoxin (TeNT) and of several botulinum neurotoxin (BoNT) types: type B, D, F, and G. The neurotoxins cause specific proteolytic degradation of the VAMP-2 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.
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基因ID
- 24803
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NCBI登录号
- NM_001330125
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UniProt
- P63045
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途径
- Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
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