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VAMP2 抗体 (N-Term)

VAMP2 适用: 人, 大鼠 WB, EIA, IHC (p) 宿主: 小鼠 Monoclonal 3-00E-005 unconjugated
产品编号 ABIN317061
发货至: 中国
  • 抗原 See all VAMP2 抗体
    VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))
    抗原表位
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-89, N-Term
    适用
    • 53
    • 34
    • 28
    • 1
    • 1
    • 1
    • 1
    人, 大鼠
    宿主
    • 46
    • 7
    • 3
    • 1
    • 1
    小鼠
    克隆类型
    • 39
    • 19
    单克隆
    标记
    • 32
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    This VAMP2 antibody is un-conjugated
    应用范围
    • 28
    • 20
    • 10
    • 10
    • 9
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    This antibody recognizes specifically Synaptobrevin 2, but it also shows a low affinity to Synaptobrevin 1. Detects a band of approximately 14 kDa in rat brain lysates.
    产品特性
    Synonyms: VAMP2, Vesicle-associated membrane protein 2, SYB2
    纯化方法
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    免疫原
    Recombinant Human Synaptobrevin 2 (1-89 aa) purified from E. coli.
    克隆位点
    3-00E-005
    亚型
    IgG1
    Top Product
    Discover our top product VAMP2 Primary Antibody
  • 应用备注
    ELISA. Western blot (1/2,000-1/3,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated foruse in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))
    别名
    VAMP-2 / Synaptobrevin-2 (VAMP2 产品)
    别名
    zgc:73302 antibody, wu:fj61c06 antibody, VAMP2 antibody, vamp2-a antibody, xsybi antibody, Syb-2 antibody, Syb2 antibody, sybII antibody, RATVAMPB antibody, RATVAMPIR antibody, SYB antibody, SYB2 antibody, VAMP-2 antibody, syb2 antibody, vamp2 antibody, xsybii antibody, vesicle-associated membrane protein 2 antibody, vesicle associated membrane protein 2 antibody, vesicle associated membrane protein 2 S homeolog antibody, vesicle-associated membrane protein 2 (synaptobrevin 2) antibody, vesicle associated membrane protein 2 L homeolog antibody, vamp2 antibody, VAMP2 antibody, vamp2.S antibody, Vamp2 antibody, vamp2.L antibody
    背景
    Synaptobrevin 2 (Syb2) also known as vesicle associated membrane protein-2 (VAMP2) is a membrane protein that belongs to the v-SNARE family of proteins, and is an essential component in neurotransmitter exocytosis from the presynaptic membrane. It is expressed on the synaptic vesicle, where it is bound by synaptophysin. Synaptophysin targets the vesicle to the presynaptic membrane and releases Syb2, which forms a stable complex with syntaxin and SNAP-25 (both expressed on the target membrane). Nerve cell depolarization and influx of calcium leads to vesicle fusion and neurotransmitter release into the synapse.Synonyms: SYB2, VAMP2, Vesicle-associated membrane protein 2
    基因ID
    6844
    UniProt
    P63027
    途径
    Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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