VAMP2 抗体 (Cytosolic)
Quick Overview for VAMP2 抗体 (Cytosolic) (ABIN7043878)
抗原
See all VAMP2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 2-27, Cytosolic
-
原理
- A Rabbit Polyclonal Antibody to VAMP-2
-
特异性
- Cytoplasmic, N-terminus
-
交叉反应
- 小鼠, 大鼠
-
预测反应
- Human,bovine,Macaca mulata - 25,26 amino acid residues identical
-
产品特性
- Anti-v Antibody is directed against the intracellular N-terminal domain of rat V. Anti-V Antibody (ABIN7043878, ABIN7044653 and ABIN7044654) can be used in western blot and immunohistochemical applications, and has been designed to recognize V from rat, mouse and human samples.
-
纯化方法
- The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
-
免疫原
-
Immunogen: GST fusion protein
Immunogen Sequence: GST fusion protein with the sequence SATAATVPPAAPAGEGGPPAPPPNLT, corresponding to amino acid residues 2-27 of rat VAMP2
-
亚型
- IgG
-
-
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-100) antibody
VAMP2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89), (N-Term) antibodyVAMP2 适用: 人, 大鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3-00E-005 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 2-17) antibodyVAMP2 适用: 人, 小鼠, 大鼠, Hamster WB, IHC, ICC, IP 宿主: 豚鼠 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) antibodyVAMP2 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人, 小鼠 WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (N-Term) antibodyVAMP2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-94) antibodyVAMP2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-66) antibodyVAMP2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
-
-
应用备注
-
Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:1000
-
说明
-
Negative Control: (ABIN7566008)
Blocking Peptide: (ABIN7566008)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))
-
别名
- VAMP2
-
背景
-
Vesicle-associated membrane protein 2, Synaptobrevin 2, Syb2,VAMP-2 (also known as synaptobrevin-2) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionary conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.VAMP-2, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located on the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.VAMP-2 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as the vesicle membrane protein v-SNARE, which together with the plama membrane t-SNARE protein Syntaxin 1 and the membrane-associated SNAP-25 (synaptosome-associated protein 25 kDa), forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.VAMP-2 is the target of the tetanus neurotoxin (TeNT) and of several botulinum neurotoxin (BoNT) types: type B, D, F, and G. The neurotoxins cause specific proteolytic degradation of the VAMP-2 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.
Alternative names: VAMP-2, Vesicle-associated membrane protein 2, Synaptobrevin 2, Syb2 -
基因ID
- 24803
-
NCBI登录号
- NM_001330125
-
UniProt
- P63045
-
途径
- Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
-
