TPCN2 抗体 (2nd Lumenal Region)
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Quick Overview for TPCN2 抗体 (2nd Lumenal Region) (ABIN7043872)
抗原
See all TPCN2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- 2nd Lumenal Region, AA 187-199
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原理
- A Rabbit Polyclonal Antibody to Two Pore Calcium Channel Protein 2
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,rat,human - 12,13 amino acid residues identical
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产品特性
- Anti-TPCN2 Antibody (ABIN7043872, ABIN7044037 and ABIN7044038) is a highly specific antibody directed against an epitope of the mouse Two pore calcium channel protein 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TPCN2 from mouse, rat, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- KKTLKSIRW(S)LPE(C), corresponding to amino acid residues 187-199 of mouse TPCN2 with replacement of amino acid 192 with serine (S)
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亚型
- IgG
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anti-Two Pore Segment Channel 2 (TPCN2) (C-Term) antibody
Verified TPCN2 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7237543)
Blocking Peptide: (ABIN7237543)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- TPCN2 (Two Pore Segment Channel 2 (TPCN2))
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别名
- TPCN2
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背景
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Synonyms: Two pore calcium channel protein 2, Voltage-dependent calcium channel protein TPC2, SHEP10
Description: Among various vertebrate species, three genes are known to encode two-pore segment channels (TPCs) termed TPC1-3. Interestingly TPC3 seems to be absent from the genomes of primates and rodents1. The primary sequence of these channels indicates the presence of two putative pore-forming repeats. Each repeat contains six transmembrane domains and a pore loop, a structure strikingly reminiscent of many voltage-gated Na+ (NaV) and Ca2+ (CaV) channels2. These twelve transmembrane structures are further thought to form functional dimers3. Both TPC1 and TPC2 show ubiquitous expression, while that of TPC1 is exceptionally high in spleen, lung, liver, and kidney2.Ca2+-mobilizing messengers such as inositol triphosphate, cyclic ADP ribose and nicotinic acid adenosine dinucleotide phosphate (NAADP) are responsible for the intracellular changes in Ca2+ ion concentration4.In contrast to the other Ca2+-mobilizing agents, NAADP, the most potent of these Ca2+ releasing molecules increases the cytosolic Ca2+ concentration via Ca2+ channels located on acidic vesicles (endolysosomes)5,6. Only quite recently, after almost a decade of being cloned, TPC1 and TPC2 were both found to be responsible for the NAADP-induced release of Ca2+ 7,8. Evidence that these two channels are indeed responsible for the release of Ca2+ is quite compelling since overexpression of TPC1 and its knockdown or point mutation of a critical residue increase and exacerbate Ca2+ release respectively7. In addition, b-cells from TPC2 knockout mice exhibited no Ca2+ release from endolysosomes upon NAADP stimulation8. Finally, in a study using immunopurified channels, it was demonstrated that TPC1 and TPC2 both respond to very low concentrations of NAADP and are unequivocally responsible for the release of Ca2+, whereas TPC3 may negatively regulate the release of Ca2+ 9.As these channels have only recently been discovered, very little is known about their physiology and gating mechanisms. Their probable involvement in a number of diseases such as lysosomal storage disease (LSDs), caused by the dysfunction of lysosomal associated proteins, has yet to be deciphered1.
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基因ID
- 233979
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NCBI登录号
- NM_139075
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UniProt
- Q8BWC0
抗原
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