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- 抗原 See all TPCN2 抗体
- TPCN2 (Two Pore Segment Channel 2 (TPCN2))
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抗原表位
- AA 187-199
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TPCN2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- A Rabbit Polyclonal Antibody to Two Pore Calcium Channel Protein 2
- 特异性
- 2nd luminal loop
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Mouse,rat,human - 12,13 amino acid residues identical
- 产品特性
- Anti-TPCN2 Antibody (ABIN7043872, ABIN7044037 and ABIN7044038)) is a highly specific antibody directed against an epitope of the mouse Two pore calcium channel protein 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TPCN2 from mouse, rat, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: KKTLKSIRW(S)LPE(C), corresponding to amino acid residues 187-199 of mouse TPCN2 with replacement of amino acid 192 with serine (S)
- 亚型
- IgG
- Top Product
- Discover our top product TPCN2 Primary Antibody
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anti-Two Pore Segment Channel 2 (TPCN2) (C-Term) antibody
Verified TPCN2 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Two Pore Segment Channel 2 (TPCN2) (AA 11-85) antibodyTPCN2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: BLP-CC072
Blocking Peptide: BLP-CC072
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- TPCN2 (Two Pore Segment Channel 2 (TPCN2))
- 别名
- TPCN2 (TPCN2 产品)
- 背景
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Two pore calcium channel protein 2, Voltage-dependent calcium channel protein TPC2, SHEP10,Among various vertebrate species, three genes are known to encode two-pore segment channels (TPCs) termed TPC1-3. Interestingly TPC3 seems to be absent from the genomes of primates and rodents1. The primary sequence of these channels indicates the presence of two putative pore-forming repeats. Each repeat contains six transmembrane domains and a pore loop, a structure strikingly reminiscent of many voltage-gated Na+ (NaV) and Ca2+ (CaV) channels2. These twelve transmembrane structures are further thought to form functional dimers3. Both TPC1 and TPC2 show ubiquitous expression, while that of TPC1 is exceptionally high in spleen, lung, liver, and kidney2.Ca2+-mobilizing messengers such as inositol triphosphate, cyclic ADP ribose and nicotinic acid adenosine dinucleotide phosphate (NAADP) are responsible for the intracellular changes in Ca2+ ion concentration4.In contrast to the other Ca2+-mobilizing agents, NAADP, the most potent of these Ca2+ releasing molecules increases the cytosolic Ca2+ concentration via Ca2+ channels located on acidic vesicles (endolysosomes)5,6. Only quite recently, after almost a decade of being cloned, TPC1 and TPC2 were both found to be responsible for the NAADP-induced release of Ca2+ 7,8. Evidence that these two channels are indeed responsible for the release of Ca2+ is quite compelling since overexpression of TPC1 and its knockdown or point mutation of a critical residue increase and exacerbate Ca2+ release respectively7. In addition, b-cells from TPC2 knockout mice exhibited no Ca2+ release from endolysosomes upon NAADP stimulation8. Finally, in a study using immunopurified channels, it was demonstrated that TPC1 and TPC2 both respond to very low concentrations of NAADP and are unequivocally responsible for the release of Ca2+, whereas TPC3 may negatively regulate the release of Ca2+ 9.As these channels have only recently been discovered, very little is known about their physiology and gating mechanisms. Their probable involvement in a number of diseases such as lysosomal storage disease (LSDs), caused by the dysfunction of lysosomal associated proteins, has yet to be deciphered1.
Alternative names: TPCN2, Two pore calcium channel protein 2, Voltage-dependent calcium channel protein TPC2, SHEP10 - 基因ID
- 233979
- NCBI登录号
- NM_139075
- UniProt
- Q8BWC0
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