TMEM119 抗体 (Extracellular)
Quick Overview for TMEM119 抗体 (Extracellular) (ABIN7043859)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 53-67, Extracellular
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原理
- A Rabbit Polyclonal Antibody to TMEM119 (extracellular)
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特异性
- Extracellular, N-terminus.
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- This antibody will not recognize human TMEM119.
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预测反应
- Rat - 12,15 amino acid residues identical
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产品特性
- Anti-TMEM119 (extracellular) Antibody (ABIN7043859, ABIN7044740 and ABIN7044741) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119
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亚型
- IgG
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anti-Transmembrane Protein 119 (TMEM119) (AA 53-67), (Extracellular) antibody (FITC)
TMEM119 适用: 小鼠 FACS 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7237517)
Blocking Peptide: (ABIN7237517)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- TMEM119 (Transmembrane Protein 119 (TMEM119))
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别名
- TMEM119
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背景
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Transmembrane Protein 119, Osteoblast induction factor, OBIF,Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.
Alternative names: Transmembrane Protein 119, Osteoblast induction factor, OBIF -
基因ID
- 231633
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NCBI登录号
- NM_181724
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UniProt
- Q8R138
抗原
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