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S1PR3 抗体 (Extracellular)

Name: S1PR3 抗体 (Extracellular)
SKU: ABIN7043774
Availability: OutOfStock

This anti-S1PR3 antibody is a 兔多克隆 antibody detecting S1PR3 in WB, FACS 和 LCI. Suitable for 小鼠.

Western blot analysis of rat heart (lanes 1 and 3), rat kidney (lanes 2 and 4), human Jurkat acute T cell leukemia (lanes 5 and 7) and mouse WEHI B cell lymphoma (lanes 6 and 8) lysates: - 1,2,5,6. (S1PR3 抗体 (Extracellular))

Cell surface detection of S1PR3 in Raji (human Burkitt's lymphoma) living cells: (black line) Unstained cells. (S1PR3 抗体 (Extracellular))

FACS

2 images

产品编号 ABIN7043774

发货至: 中国

Quick Overview for S1PR3 抗体 (Extracellular) (ABIN7043774)

抗原

See all S1PR3 抗体

S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

适用

小鼠

宿主

兔

克隆类型

多克隆

标记

This S1PR3 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Discover our top product S1PR3 Primary Antibody

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AA 23-34, Extracellular

原理

A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3

特异性

Extracellular, N-terminus

交叉反应

人, 小鼠, 大鼠

预测反应

Rat - identical, human - 9,12 amino acid residues identical

产品特性

Anti-S1PR3 (EDG3) (extracellular) Antibody (ABIN7043774, ABIN7045274 and ABIN7045275) is a highly specific antibody directed against an extracellular epitope of mouse sphingosine 1-phosphate receptor 3. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize S1PR3 from human, rat and mouse samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

Immunogen: Synthetic peptide
Immunogen Sequence: (C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse S1PR3

亚型

IgG
anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 1-378) antibody
S1PR3 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 303-378) antibody
S1PR3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 145-250) antibody
S1PR3 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (N-Term) antibody
S1PR3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 201-250) antibody
S1PR3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 302-378) antibody
S1PR3 适用: 人 ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2G11 unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 12-25) antibody
S1PR3 适用: 人 WB, IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (N-Term) antibody
S1PR3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (AA 300-350) antibody
S1PR3 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine-1-Phosphate Receptor 3 (S1PR3) (Internal Region) antibody
Verified S1PR3 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

应用备注

Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200

说明

Cited Application: IFC
Negative Control: (ABIN7237275)
Blocking Peptide: (ABIN7237275)

限制

仅限研究用
状态

Lyophilized

溶解方式

Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

浓度

1 mg/mL

缓冲液

PBS pH 7.4

储存条件

4 °C,-20 °C

储存方法

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
抗原

S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

别名

S1PR3

背景

Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3,Sphingosine 1-phosphate (S1P) is an active byproduct of sphingomyelin metabolism. All cells can synthesize this biomolecule but the majority of its synthesis comes from erythrocytes and endothelial cells. In cases of inflammation, mast cells and platelets are the main source of sphingosine 1-phosphate1. S1P cellular functions could be intracellular, where it is synthesized. In addition, upon secretion, it circulates in the blood via its binding to high-density lipoproteins and albumin. When S1P reaches its target cell it could activate 5 different high affinity receptors belonging to the G-protein coupled receptor superfamily: Sphingosine 1-phosphate receptors, termed S1PR1-51.Stimulation of S1P receptors triggers a cascade of signaling events depending on the receptor and on the G-protein it couples. S1PR1 couples to Gi2. S1PR2 can couple to Gs, Gq or G12/13 and S1PR3-5 can couple to Gior G12/133-4. The pathways activated vary from Ca2+ mobilization, activation or inhibition of adenylate cyclase phospholipase C activation and more. Through the different signaling pathways these receptors activate, S1P1 receptors are implicated in adherens junction assembly, cytoskeletal changes, cell migration, proliferation and apoptosis5.S1PR3 is specifically detected in the brain, heart, spleen, liver, lung, thymus, kidney, testis and skeletal muscle3. S1PR1 and S1PR2 are generally expressed in the CNS, cardiovascular and immune systems1. S1PR4 is specifically expressed in the lymphoid tissue and S1PR5 in natural killer cells and olygodendrocytes1.Malfunction of S1P receptor signaling is reported in various disorders, for example multiple sclerosis and may be targets for the development of therapeutic drugs1.

Alternative names: S1PR3 (EDG3), Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3

基因ID

13610

NCBI登录号

NM_005226

UniProt

Q9Z0U9