Sortilin 1 抗体 (Extracellular)
Quick Overview for Sortilin 1 抗体 (Extracellular) (ABIN7043768)
抗原
See all Sortilin 1 (SORT1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 320-335, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Sortilin
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特异性
- Extracellular domain
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,rat - identical
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产品特性
- Anti-Sortilin (extracellular) Antibody (ABIN7043768, ABIN7044759 and ABIN7044760) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize Sortilin from mouse, rat, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin
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亚型
- IgG
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anti-Sortilin 1 (SORT1) (AA 203-299) antibody
SORT1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B3 unconjugated
anti-Sortilin 1 (SORT1) (AA 520-760) antibodySORT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sortilin 1 (SORT1) (AA 328-457) antibodySORT1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sortilin 1 (SORT1) (AA 731-831) antibodySORT1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sortilin 1 (SORT1) (AA 50-320) antibodySORT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sortilin 1 (SORT1) (AA 203-299) antibodySORT1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Sortilin 1 (SORT1) antibodyKD Validated SORT1 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-Sortilin 1 (SORT1) (AA 320-335), (Extracellular) antibody (FITC)SORT1 适用: 人 FACS 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:400
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说明
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Cited Application: IP|IHC
Negative Control: (ABIN7237255)
Blocking Peptide: (ABIN7237255)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Sortilin 1 (SORT1)
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别名
- SORT1
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背景
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SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95,Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has lately been identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in the brain, spinal cord, skeletal muscle and adipocytes.Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90 % of sortilin is retained in intracellular compartments with only about 10 % reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.
Alternative names: Sortilin, SORT1, Neurotensin Receptor 3, NTR3, NT3, Glycoprotein 95, Gp95 -
基因ID
- 6272
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NCBI登录号
- NM_002959
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UniProt
- Q99523
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途径
- Neurotrophin Signaling Pathway
抗原
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