SLC7A11 抗体 (Extracellular) (PE)
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Quick Overview for SLC7A11 抗体 (Extracellular) (PE) (ABIN7043753)
抗原
See all SLC7A11 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 218-231, Extracellular
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原理
- A Rabbit Polyclonal Antibody to xCT/SLC7A11 Conjugated to the Fluorescent Dye R-Phycoerythrin
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特异性
- 3rd extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat - identical, human - 11,14 amino acid residues identical
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产品特性
- Anti-xCT/SLC7A11 (extracellular) Antibody (ABIN7043751, ABIN7044835 and ABIN7044836) is a highly selective antibody directed against an epitope of mouse cystine/glutamate transporter. The antibody can be used in western blot analysis. It has been designed to recognize xCT from human, mouse, and rat samples. \nAnti-xCT/SLC7A11 (extracellular)-PE Antibody (ABIN7043753, ABIN7045636, ABIN7045637, ABIN7045638 and ABIN7045639) is directly conjugated to Phycoerythrin (PE). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse xCT/SLC7A11
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亚型
- IgG
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anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) (Internal Region) antibody
Verified SLC7A11 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) antibodySLC7A11 适用: 人 WB, IHC, ELISA, IP, FACS, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) antibodySLC7A11 适用: 人 WB, IHC, ELISA, IP, FACS, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) (AA 218-231), (Extracellular) antibodySLC7A11 适用: 小鼠 WB, IHC, FACS, IF, LCI 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) (AA 201-300) antibodySLC7A11 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) antibodySLC7A11 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) antibodySLC7A11 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB2215 unconjugated Recombinant Antibody
anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) (AA 215-264) antibodySLC7A11 适用: 人, 小鼠, 大鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) (AA 1-50), (N-Term) antibodySLC7A11 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582046)
Blocking Peptide: (ABIN7237192)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 15 μL or 50 μL double distilled water (DDW), depending on the sample size.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))
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别名
- SLC7A11
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背景
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Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1,The SLC7 family is divided into two subgroups, the cationic amino acid transporters (the CAT family, SLC7A1-4) and the glycoprotein-associated amino acid transporters (the gpaAT family, SLC7A5-11) also called light chains or catalytic chains of the hetero(di)meric amino acid transporters (HAT). The gene SLC7A11 encodes the xCT protein (also known as Cystine/glutamate transporter).xCT, as well as other HATs, is comprised of a light chain with 12 non-glycosylated transmembrane helices and is associated with the glycosylated heavy chain 4F2hc (CD98, SLC3A2) or rBAT (SLC3A1) through a conserved disulphide bridge. The COOH-terminus of the xCT is localized intracellularly. Association of the light and heavy chains is required for surface expression of the transporter which forms the heterodimeric amino acid transport system xc-. xCT is mainly expressed macrophages, brain, retinal pigment cells, liver and kidney. xCT expression is elevated in cells requiring high glutathione synthesis, such as activated macrophages, neuronal and glial cells and other cells after glutathione depletion1.xCT operates in a Na+-independent and electroneutral mode, exchanging extracellular anionic cystine (with pH dependence) for glutamate with a stoichiometry of 1:1. The driving force for this exchange is generated by the cystine concentration gradient (intracellular reduction) and the high intracellular concentration of glutamate.xCT has been suggested as a mediator of treatment resistance in cancer patients. In tumor cells, xCT plays a crucial role in regulating intracellular levels of glutathione which has been broadly implicated in resistance to chemotherapy2.
Alternative names: Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1 -
基因ID
- 26570
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NCBI登录号
- NM_014331
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UniProt
- Q9WTR6
抗原
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