SLC7A11 抗体
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- 抗原 See all SLC7A11 抗体
- SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SLC7A11 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
- 纯化方法
- This affinity purified antibody is directed against human xCT. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and orangutan, and 88% identity to mouse.
- 免疫原
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Immunogen: Anti-xCT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human xCT conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product SLC7A11 Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: 1:200
Application Note: Anti-xCT Antibody has been tested in Western Blot, ELISA, Immunofluorescence, and flow cytometry. Expect a band at 55.4 kDa in western blot using appropriate lysates. Positive control used HeLa, NIH-3T3, A549, HCT-116, or PC3 whole cell lysates at 0.1-1.0 μg/mL for WB and PC3 with MeOH at 10 μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 5 μg/mL
Flow Cytometry Dilution: 1:200
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 10 μg/mL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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- 抗原
- SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))
- 别名
- xCT (SLC7A11 产品)
- 别名
- SLC7A11 antibody, DKFZp468E122 antibody, CCBR1 antibody, xCT antibody, 9930009M05Rik antibody, AI451155 antibody, sut antibody, solute carrier family 7 member 11 antibody, solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody, solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 antibody, SLC7A11 antibody, Slc7a11 antibody
- 背景
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Synonyms: rabbit anti-xCT antibody, SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11
Background: xCT belongs to the amino acid-polyamine-organocation (APC) superfamily and L-type amino acid transporter (LAT) family. xCT gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. Increased expression of this gene results in neuronal cell death. xCT may be associated with kaposi sarcoma, dyscalculia, cystinuria, spondylolsis, and anemia of prematurity. Anti-xCT Antibody is useful for researchers interested in Cancer Research, Glucose/Energy Metabolism Research, and Cell Surface Protein Interaction Research.
Gene Name: SLC7A11
- 基因ID
- 23657
- NCBI登录号
- NP_055146
- UniProt
- Q9UPY5
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