SLC1A5 抗体 (Extracellular)
Quick Overview for SLC1A5 抗体 (Extracellular) (ABIN7043669)
抗原
See all SLC1A5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 202-217, Extracellular
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原理
- A Rabbit Polyclonal Antibody to ASCT2 Transporter
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特异性
- 2nd extracellular loop
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交叉反应 （详细）
- The antibody will not recognize rat or mouse ASCT2.
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预测反应
- Human only
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产品特性
- Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (ABIN7043669, ABIN7044825 and ABIN7044826) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RSYSTTYEERNITGTR, corresponding to amino acid residues 202 - 217 of human ASCT2
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亚型
- IgG
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anti-Solute Carrier Family 1 Member 5 (SLC1A5) (Internal Region) antibody
SLC1A5 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 Member 5 (SLC1A5) antibodySLC1A5 适用: 人, 小鼠, 大鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 Member 5 (SLC1A5) (Middle Region) antibodySLC1A5 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, Pig, 绵羊, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 Member 5 (SLC1A5) (AA 1-100) antibodySLC1A5 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 Member 5 (SLC1A5) (AA 241-290) antibodySLC1A5 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, Pig, 绵羊, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 Member 5 (SLC1A5) antibodySLC1A5 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 Member 5 (SLC1A5) antibodySLC1A5 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 Member 5 (SLC1A5) antibodySLC1A5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 Member 5 (SLC1A5) (Internal Region) antibodySLC1A5 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))
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别名
- SLC1A5
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背景
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Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2,ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.
Alternative names: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2, ASCT2, SLC1A5 -
基因ID
- 6510
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NCBI登录号
- NM_001145144
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UniProt
- Q15758
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途径
- Dicarboxylic Acid Transport, Warburg Effect
抗原
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