ROBO1 抗体 (Extracellular)
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Quick Overview for ROBO1 抗体 (Extracellular) (ABIN7043603)
抗原
See all ROBO1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 491-506, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Robo1
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,rat - identical
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产品特性
- Anti-Robo1 (extracellular) Antibody (ABIN7043603, ABIN7044744 and ABIN7044745) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize Robo1 from human, rat, and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)DGVLVSTQDSRIKQLE, corresponding to amino acid residues 491 - 506 of human Robo1
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亚型
- IgG
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anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (Internal Region) antibody
Verified ROBO1 适用: 人 ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (AA 1625-1650) antibodyROBO1 适用: 人, 小鼠 WB, ELISA, IHC, IP, FM 宿主: 兔 Polyclonal unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (AA 1452-1651) antibodyROBO1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (AA 29-143) antibodyROBO1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (Internal Region) antibodyVerified ROBO1 适用: 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (AA 491-589) antibodyROBO1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (AA 1097-1130) antibodyROBO1 适用: 人 WB 宿主: 兔 Polyclonal RB54414 unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (AA 491-589) antibodyROBO1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (Internal Region) antibodyROBO1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) (AA 491-506), (Extracellular) antibody (FITC)ROBO1 适用: 人 FACS, LCI 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7236733)
Blocking Peptide: (ABIN7236733)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
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别名
- ROBO1
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背景
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Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1,Roundabout (Robo) receptors are a conserved family of four single pass transmembrane proteins1. All members have multiple immunoglobulin-like domains in the extracellular N-terminal region, three extracellular fibronectin type II domains, and cytoplasmic conserved domains. Robos also undergo preoteolytic processing, important for their signaling process2. They serve as receptors for Slit proteins, where upon activation lead to pleiotropic effects including axonal guidance1, axonal repulsion3,4, and neural migration5.Slit/Robo signaling mainly prevents axons from migrating to inappropriate locations during the assembly of the nervous system6.Robos are mainly expressed in the nervous system, but are also detected in vascular endothelial and muscle cells7. In non-neuronal tissues, Slit/Robo proteins are key oncogenes and tumor suppressors in various types of cancers. Robo1 expression is elevated in breast cancer and colorectal cancer, and has an overall oncogenic role1.
Alternative names: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1 -
基因ID
- 6091
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NCBI登录号
- NM_002941
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UniProt
- Q9Y6N7
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途径
- Positive Regulation of Endopeptidase Activity
抗原
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