P2RX7 抗体 (Extracellular) (Atto 488)
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Quick Overview for P2RX7 抗体 (Extracellular) (Atto 488) (ABIN7043579)
抗原
See all P2RX7 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 136-152, Extracellular
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原理
- A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye ATTO-488
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特异性
- Extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Human,rat - identical, bovine - 14,17 amino acid residues identical
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产品特性
- Anti-P2X7 Receptor (extracellular) Antibody (ABIN7043578, ABIN7045080 and ABIN7045081) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples. \nAnti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (ABIN7043579) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor
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亚型
- IgG
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anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (AA 226-452) antibody
P2RX7 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7G1D6 unconjugated
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (Center) antibodyP2RX7 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (AA 136-152), (Extracellular) antibody (FITC)KO Validated P2RX7 适用: 小鼠 IF, FACS, LCI, IC 宿主: 兔 Polyclonal FITC
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (N-Term) antibodyP2RX7 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (AA 136-152), (Extracellular) antibodyKO Validated P2RX7 适用: 小鼠 WB, IHC, IF, FACS, LCI, IC 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (AA 576-595), (Intracellular) antibody (Atto 550)KO Validated P2RX7 适用: 大鼠 IHC, IF 宿主: 兔 Polyclonal Atto 550
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (AA 47-334) antibodyP2RX7 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (N-Term) antibodyVerified P2RX7 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (C-Term) antibodyP2RX7 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7) (Internal Region) antibodyP2RX7 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:25
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说明
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Cited Application: FC
Negative Control: (ABIN7582041)
Blocking Peptide: (ABIN7236676)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))
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别名
- P2RX7
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背景
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Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor,The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4
Alternative names: P2X7 Receptor, Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor -
基因ID
- 18439
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NCBI登录号
- NM_002562
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UniProt
- Q9H244
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis
抗原
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