Nerve Growth Factor 抗体 (Pro-Domain)
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Quick Overview for Nerve Growth Factor 抗体 (Pro-Domain) (ABIN7043555)
抗原
See all Nerve Growth Factor (NGF) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 84-104, Pro-Domain
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原理
- A Guinea Pig Polyclonal Antibody to proNGF
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 （详细）
- The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.
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预测反应
- Mouse - identical, human - 16,21 amino acid residues identical
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产品特性
- Guinea pig Anti-proNGF Antibody is directed against rat proNGF. Guinea pig Anti-proNGF Antibody can be used in western blot and immunohistochemistry applications. Anti-proNGF Antibody raised in guinea pig recognizes the same epitope as Anti-proNGF Antibody (ABIN7043556, ABIN7044751 and ABIN7044752) raised in rabbit. The antibody specifically reacts with proNGF and does not crossreact with proBDNF, pro or mature NGF. It has been designed to recognize proNGF from mouse, rat and human samples. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc. \nRelated products for neutralizing proNGF activity: \nMouse NGF/proNGF Neutralizing Antibody can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)
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亚型
- IgG
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anti-Nerve Growth Factor (NGF) antibody
NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibodyNGF 适用: 小鼠 ELISA, Neut 宿主: 小鼠 Monoclonal HAL06 unconjugated
anti-Nerve Growth Factor (NGF) (AA 19-241) antibodyNGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 162-173), (N-Term) antibodyNGF 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (N-Term) antibodyNGF 适用: 人, 大鼠, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibody (Biotin)NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Nerve Growth Factor (NGF) (AA 122-241) antibodyNGF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 122-218) antibodyNGF 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibodyNGF 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 5F10 unconjugated Recombinant Antibody
anti-Nerve Growth Factor (NGF) (AA 19-239) antibodyNGF 适用: 人 WB, ICC, IF 宿主: 小鼠 Monoclonal C1 unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:400
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7236601)
Blocking Peptide: (ABIN7236601)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- Nerve Growth Factor (NGF)
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别名
- NGF
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背景
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Synonyms: Nerve growth factor precursor
Description: Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8
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基因ID
- 310738
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NCBI登录号
- NM_002506
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UniProt
- P25427
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途径
- Regulation of Cell Size
抗原
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