KCNJ1 抗体 (AA 342-391)
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Quick Overview for KCNJ1 抗体 (AA 342-391) (ABIN7043467)
抗原
See all KCNJ1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 342-391
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原理
- A Rabbit Polyclonal Antibody to KCNJ1 (Kir1.1) Channel
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特异性
- Intracellular, C-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 45,50 amino acid residues identical
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产品特性
- Anti-KCNJ1 (Kir1.1) Antibody (ABIN7043467, ABIN7044897 and ABIN7044898) is a highly specific antibody directed against an epitope of the rat potassium channel ROM-K. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize ROMK1 from rat, mouse, and human samples.
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纯化方法
- The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
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免疫原
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Immunogen: GST fusion protein
Immunogen Sequence: GST fusion protein with the sequence HNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDET DDTQM, corresponding to amino acids 342-391 of rat KCNJ1
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亚型
- IgG
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anti-Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1) antibody
KCNJ1 适用: 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1) (AA 178-391) antibodyKCNJ1 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1) (Middle Region) antibodyKCNJ1 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1) (Internal Region) antibodyVerified KCNJ1 适用: 人 ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1) (AA 292-391) antibodyKCNJ1 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1) (AA 216-265) antibodyKCNJ1 适用: 人, Cow, 犬, 豚鼠, 马, 兔, 猴, Bat, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1) (AA 11-60), (pSer44) antibodyKCNJ1 适用: 人, 大鼠, 小鼠 ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: IP
Negative Control: (ABIN7236357)
Blocking Peptide: (ABIN7236357)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- KCNJ1 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1))
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别名
- KCNJ1
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背景
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ROMK1, ATP-sensitive inward rectifier potassium channel 1,Kir1.1 (KCNJ1, ROMK1) was the first member of the family of inward rectifying K+ channels to be cloned.1 The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channel is composed of four subunits that can assemble as either homo or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.3As its original name indicates (ROMK1, Renal Outer Medullary K+ channel), Kir1.1 is strongly expressed in the kidney in the apical membrane of several kidney segments such as the thick ascending loop of Henle (TAL) and the cortical collecting duct (CCD). In addition, the channel is also expressed in the brain, mainly in the cortex and hippocampus.3Kir1.1 plays a key role in K+ recycling in the loop of Henle. Indeed, loss-of-function mutations in the Kir1.1 gene cause Bartter's syndrome type II, a recessive autosomal disease characterized by the impairment of K+ efflux and the subsequent inability of the NKCC2 transporter to continue NaCl uptake. This leads to a high salt concentration in the urine that induces osmotic diuresis and low plasma volume.2 Pharmacologically, the Kir1.1 channel can be inhibited by several general K+ channel blockers such as Tertiapin (#STT-250), however the scorpion toxin Lq2 (#RTL-550) specifically and potently inhibits Kir1.1 channels.4
Alternative names: KCNJ1 (Kir1.1), ROMK1, ATP-sensitive inward rectifier potassium channel 1 -
基因ID
- 24521
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NCBI登录号
- NM_000220
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UniProt
- P35560
抗原
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