KCNK2 抗体 (Intracellular)
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- 抗原 See all KCNK2 抗体
- KCNK2 (Potassium Channel, Subfamily K, Member 2 (KCNK2))
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抗原表位
- AA 8-25, Intracellular
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This KCNK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)
- 原理
- A Rabbit Polyclonal Antibody to KCNK2 Channel
- 特异性
- Intracellular, N-terminus
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Rat - identical, mouse - 17,18 amino acid residues identical
- 产品特性
- Anti-KCNK2 Antibody (ABIN7043452, ABIN7044957 and ABIN7044958) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize from mouse, rat, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 质量等级
- KO Validated
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2
- 亚型
- IgG
- Top Product
- Discover our top product KCNK2 Primary Antibody
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anti-Potassium Channel, Subfamily K, Member 2 (KCNK2) (AA 8-25), (Intracellular) antibody
KCNK2 适用: 人 WB, IHC, IF 宿主: 豚鼠 Polyclonal unconjugated
anti-Potassium Channel, Subfamily K, Member 2 (KCNK2) (AA 1-100), (N-Term) antibodyKCNK2 适用: 人, 小鼠, Cow WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- 说明
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Cited Application: IP|IHC|ICC
Negative Control: (ABIN7236314)
Blocking Peptide: (ABIN7236314)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- KCNK2 (Potassium Channel, Subfamily K, Member 2 (KCNK2))
- 别名
- KCNK2 (KCNK2 产品)
- 背景
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Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1,KCNK2 (also named TWIK-related K+ channel, TREK-1 or K2P2.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH , mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK2 can be integrated to a K2P subfamily that includes K2P4.1 (TRAAK) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid and mechanical stretch. In addition, KCNK2 can also be activated by general anesthetics such as halothane and chloroform and intracellular acidification.KCNK2 expression in humans is largely restricted to the brain with some expression in ovary and small intestine while KCNK2 expression in rodents is more widespread.KCNK2 has an important role in mood regulation, as knockout mice show resistance to depression, suggesting that KCNK2 may be a potential target for anti-depressants.KCNK2 is involved in the fast release of glutamate from astrocytes. It requires the activation of Gαi, dissociation of Gβγ, followed by the opening of the glutamate permeable KCNK2 (TREK-1) K+ channel through its interaction with Gβγ. For this purpose, KCNK2 is localized at the cell surface of the cell body and processes of astrocytes.
Alternative names: KCNK2 (TREK-1), Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1 - 基因ID
- 3776
- NCBI登录号
- NM_014217
- UniProt
- O95069
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