KCNK18 抗体 (Extracellular)
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- 抗原 See all KCNK18 抗体
- KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))
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抗原表位
- AA 62-75, Extracellular
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This KCNK18 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)
- 原理
- A Rabbit Polyclonal Antibody to KCNK18 Channel
- 特异性
- 1st extracellular loop
- 交叉反应
- 小鼠, 大鼠
- 交叉反应 （详细）
- Unlikely to recognize human samples.
- 预测反应
- Rat - 13,14 amino acid residues identical
- 产品特性
- Anti-KCNK18 (TRESK) (extracellular) Antibody is directed against an epitope of mouse KCNK18. Anti-KCNK18 (TRESK) (extracellular) Antibody (ABIN7043446, ABIN7045021 and ABIN7045022) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize TRESK channel from mouse and rat samples. The antibody will unlikely recognize TRESK from human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18
- 亚型
- IgG
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anti-Potassium Channel Subfamily K Member 18 (KCNK18) (AA 168-197) antibody
KCNK18 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33126 unconjugated
anti-Potassium Channel Subfamily K Member 18 (KCNK18) (Center) antibodyKCNK18 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: (ABIN7236295)
Blocking Peptide: (ABIN7236295)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))
- 别名
- KCNK18 (KCNK18 产品)
- 背景
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Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1,KCNK18 (also named TWIK-related spinal cord K+ channel, TRESK or K2P18.1) is a member of the 2-pore (2P) domain K+ channel family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.KCNK18 is the only K2P channel, known so far, whose current is activated following Gαq-receptor coupled activation. The enhancement of KCNK18 current involves activation of calcineurin (calcium-calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gαq activation.2 In addition, KCNK18 is potently activated by clinical concentrations of volatile anesthetics.3KCNK18 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.KCNK18 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.
Alternative names: KCNK18 (TRESK), Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1 - 基因ID
- 332396
- NCBI登录号
- NM_181840
- UniProt
- Q6VV64
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