ORAI1 抗体 (Extracellular)
-
- 抗原 See all ORAI1 抗体
- ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))
-
抗原表位
- Extracellular
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This ORAI1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunofluorescence (IF), Live Cell Imaging (LCI), Flow Cytometry (FACS), Immunochromatography (IC)
- 原理
- A Mouse Monoclonal Antibody to Human Orai1 Channel
- 特异性
- Extracellular
- 交叉反应 （详细）
- Recognizes only human Orai1.
- 预测反应
- Orai1 from human samples only
- 产品特性
- Mouse Anti-Human Orai1 (extracellular) Antibody (ABIN7043409 and ABIN7044554) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: Synthetic mapping to an extracellular sequence of human Orai1
- 克隆位点
- 3F11-D10-B9
- 亚型
- IgM
- Top Product
- Discover our top product ORAI1 Primary Antibody
-
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 200-212), (Extracellular) antibody
KO Validated ORAI1 适用: 小鼠 WB, IHC, IF, LCI, FACS, IC 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 1-87) antibodyORAI1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (C-Term) antibodyORAI1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (Internal Region) antibodyVerified ORAI1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 202-301) antibodyORAI1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 145-173) antibodyORAI1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) antibodyORAI1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 182-231) antibodyORAI1 适用: 人, Pig, 犬, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) antibodyORAI1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 269-298) antibodyORAI1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Antigen preadsorption control: NA
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
- 抗原
- ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))
- 别名
- ORAI1 (ORAI1 产品)
- 背景
-
TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1,Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified, Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.
Alternative names: Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1 - 基因ID
- 84876
- NCBI登录号
- NM_032790
- UniProt
- Q96D31
- 途径
- TCR Signaling, BCR Signaling
-
