ORAI1 抗体 (Extracellular)
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Quick Overview for ORAI1 抗体 (Extracellular) (ABIN7043412)
抗原
See all ORAI1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 203-214, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Human Orai1 Channel
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特异性
- 2nd extracellular loop
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交叉反应 （详细）
- Will not recognize mouse or rat Orai1.
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预测反应
- Canis - identical, bovine - 12,13 amino acid residues identical
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产品特性
- Anti-Human Orai1 (extracellular) Antibody (ABIN7043412, ABIN7044020 and ABIN7044021) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize Orai1 from human samples only.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1
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亚型
- IgG
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anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 200-212), (Extracellular) antibody
KO Validated ORAI1 适用: 小鼠 WB, IHC, FACS, IF, LCI, IC 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 202-301) antibodyORAI1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 1-87) antibodyORAI1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (Internal Region) antibodyVerified ORAI1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (C-Term) antibodyORAI1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 145-173) antibodyORAI1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (Extracellular) antibodyORAI1 适用: 人 WB, FACS, IF, LCI, IC 宿主: 小鼠 Monoclonal 3F11-D10-B9 unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) antibodyORAI1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 182-231) antibodyORAI1 适用: 人, Pig, 犬, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 269-298) antibodyORAI1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))
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别名
- ORAI1
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背景
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TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1,Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified, Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.
Alternative names: Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1 -
基因ID
- 84876
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NCBI登录号
- NM_032790
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UniProt
- Q96D31
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途径
- TCR Signaling, BCR Signaling
抗原
-
