MICU1 抗体 (Intracellular)
Quick Overview for MICU1 抗体 (Intracellular) (ABIN7043334)
抗原
See all MICU1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 313-327, Intracellular
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原理
- A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uptake Protein 1 (CBARA1)
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特异性
- Intracellular
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交叉反应
- 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 14,15 amino acid residues identical
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产品特性
- Anti-MICU1 Antibody (ABIN7043334, ABIN7044111 and ABIN7044112) is a highly specific antibody directed against an epitope of the rat Mitochondrial calcium uptake protein 1 (CBARA1). The antibody can be used in western blot analysis. It has been designed to recognize CBARA1 from rat, mouse, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1
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亚型
- IgG
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anti-Mitochondrial Calcium Uptake 1 (MICU1) antibody
MICU1 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF 宿主: 小鼠 Monoclonal 4E7 unconjugated
anti-Mitochondrial Calcium Uptake 1 (MICU1) antibodyMICU1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Mitochondrial Calcium Uptake 1 (MICU1) (AA 55-191) antibodyMICU1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Calcium Uptake 1 (MICU1) antibodyMICU1 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Calcium Uptake 1 (MICU1) (AA 113-126) antibodyVerified MICU1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Mitochondrial Calcium Uptake 1 (MICU1) antibodyMICU1 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal Mix unconjugated
anti-Mitochondrial Calcium Uptake 1 (MICU1) (AA 445-474), (C-Term) antibodyMICU1 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Calcium Uptake 1 (MICU1) (AA 380-478) antibodyMICU1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235963)
Blocking Peptide: (ABIN7235963)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))
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别名
- MICU1
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背景
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Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS,Mitochondrial Ca2+ uptake was found to be mediated by the mitochondrial Ca2+ uniporter (MCU). MCU is a ruthenium-red-sensitive and highly selective Ca2+ ion channel that uptakes massive amount of Ca2+ across the inner membrane1. MICU1 is a ~54 kDa. protein with an amino-terminal mitochondrial targeting sequence, a predicted transmembrane helix, and a cytosolic C-terminus containing two classical EF-hand Ca2+-binding domains2.Mitochondrial calcium uptake 1 (MICU1) protein was identified as an essential element of mitochondrial Ca2+ uptake. MCU initially takes up Ca2+ very rapidly, but when [Ca2+] inevitably increases inside the matrix, MICU1 binds Ca2+ through its EF-hand domains, exerting an inhibitory role on MCU-dependent Ca2+ entry3.RNA expression of MICU1 is strongly correlated across a variety of tissues including: stomach, kidney, small and large intestine, cerebral cortex, cerebellum, hypothalamus, spinal cord and skeletal muscles4.Loss-of-function mutations in MICU1 causes a brain and muscle disorder linked to primary alterations in mitochondrial Ca2+ signaling5.
Alternative names: MICU1, Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS -
基因ID
- 365567
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NCBI登录号
- NM_006077
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UniProt
- Q6P6Q9
抗原
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