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MANF 抗体 (Mature)

This anti-MANF antibody is a 兔 多克隆 antibody detecting MANF in WB, IHC 和 IF. Suitable for 人.
产品编号 ABIN7043330
发货至: 中国

Quick Overview for MANF 抗体 (Mature) (ABIN7043330)

抗原

See all MANF 抗体
MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

适用

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宿主

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克隆类型

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多克隆

标记

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This MANF antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
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    AA 91-104, Mature

    原理

    A Rabbit Polyclonal Antibody to MANF

    特异性

    Mature MANF protein

    交叉反应

    人, 小鼠, 大鼠

    预测反应

    Rat,mouse - identical

    产品特性

    Anti-MANF/ARMET Antibody (ABIN7043330, ABIN7044787 and ABIN7044788) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MANF from human, rat and mouse samples.

    纯化方法

    Affinity purified on immobilized antigen.

    免疫原

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF

    亚型

    IgG
  • 应用备注

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

    Application Dilutions Western blot wb: 1:200

    说明

    Cited Application: IHC

    Negative Control: (ABIN7235952)

    Blocking Peptide: (ABIN7235952)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4

    储存条件

    4 °C,-20 °C

    储存方法

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • 抗原

    MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

    别名

    MANF

    背景

    Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP,The novel protein mesencephalic astrocyte-derived neurotrophic factor (MANF) is a survival molecule (alternatively referred to as arginine-rich, mutated in early stage tumors, or ARMET) that has high selectivity for dopaminergic neurons of the midbrain1,2. There, it induces proliferative and protective effects against neurodegeneration (usually caused by 6-hydroxydopamine), its therapeutic potential in dopamine-related neurodegenerative disorders such as Parkinson's disease is therefore promising3,4.MANF is also expressed cardiac tissues. As was recently suggested, it induces cardioprotection in the heart and anti-hypertrophic effects via reducing endoplasmic reticulum stress in ischemic conditions in both the heart and the brain. Thus, its effects stretch beyond rescue and protection of dopaminergic neurons alone1,5.Generally, neurotrophic factors (NTFs) provide support, protection, and regulation for neurons - with each group of neurons having their own unique NTFs - both during development and at adulthood. Other noticeable NTFs include neurotrophin-3 and -4 (NT3 and NT4), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), and glial cell derived neurotrophic factor (GDNF)6.MANF is considered to be evolutionary conserved within Mammalia and beyond (including distant species such as nematode and fruit fly - the latter shares more than 50 % identity with human MANF gene) and is highly mutative in many tumors. It is mostly composed of alpha helices and random coils, its structure essentially differs from any other neurotrophic factor. It is suggested that the protein initiates intracellular signaling cascade by binding to cell-surface lipids and membranes via its cysteine-rich N-terminal. Together with its C-terminal which contains a disulfide bridge that endows a cytoprotective response against ER stress, MANF is implied to be bi-functional3,7.

    Alternative names: MANF/ARMET, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP

    基因ID

    7873

    NCBI登录号

    NM_006010

    UniProt

    P55145
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