MANF 抗体 (Mature)
Quick Overview for MANF 抗体 (Mature) (ABIN7043330)
抗原
See all MANF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 91-104, Mature
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原理
- A Rabbit Polyclonal Antibody to MANF
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特异性
- Mature MANF protein
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,mouse - identical
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产品特性
- Anti-MANF/ARMET Antibody (ABIN7043330, ABIN7044787 and ABIN7044788) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MANF from human, rat and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF
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亚型
- IgG
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anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 25-182) antibody
MANF 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 28-182) antibodyMANF 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (C-Term) antibodyKO Validated MANF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 116-185) antibodyMANF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1D10 unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 25-182) antibodyMANF 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) antibodyMANF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) antibodyMANF 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (Internal Region) antibodyVerified MANF 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 1-182) antibodyMANF 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 103-152) antibodyMANF 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, 斑马鱼, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: IHC
Negative Control: (ABIN7235952)
Blocking Peptide: (ABIN7235952)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
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别名
- MANF
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背景
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Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP,The novel protein mesencephalic astrocyte-derived neurotrophic factor (MANF) is a survival molecule (alternatively referred to as arginine-rich, mutated in early stage tumors, or ARMET) that has high selectivity for dopaminergic neurons of the midbrain1,2. There, it induces proliferative and protective effects against neurodegeneration (usually caused by 6-hydroxydopamine), its therapeutic potential in dopamine-related neurodegenerative disorders such as Parkinson's disease is therefore promising3,4.MANF is also expressed cardiac tissues. As was recently suggested, it induces cardioprotection in the heart and anti-hypertrophic effects via reducing endoplasmic reticulum stress in ischemic conditions in both the heart and the brain. Thus, its effects stretch beyond rescue and protection of dopaminergic neurons alone1,5.Generally, neurotrophic factors (NTFs) provide support, protection, and regulation for neurons - with each group of neurons having their own unique NTFs - both during development and at adulthood. Other noticeable NTFs include neurotrophin-3 and -4 (NT3 and NT4), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), and glial cell derived neurotrophic factor (GDNF)6.MANF is considered to be evolutionary conserved within Mammalia and beyond (including distant species such as nematode and fruit fly - the latter shares more than 50 % identity with human MANF gene) and is highly mutative in many tumors. It is mostly composed of alpha helices and random coils, its structure essentially differs from any other neurotrophic factor. It is suggested that the protein initiates intracellular signaling cascade by binding to cell-surface lipids and membranes via its cysteine-rich N-terminal. Together with its C-terminal which contains a disulfide bridge that endows a cytoprotective response against ER stress, MANF is implied to be bi-functional3,7.
Alternative names: MANF/ARMET, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP -
基因ID
- 7873
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NCBI登录号
- NM_006010
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UniProt
- P55145
抗原
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