MC1 Receptor 抗体 (AA 217-232)
Quick Overview for MC1 Receptor 抗体 (AA 217-232) (ABIN7043321)
抗原
See all MC1 Receptor (MC1R) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 217-232
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原理
- A Rabbit Polyclonal Antibody to Melanocortin Receptor 1
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特异性
- 3rd intracellular loop
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交叉反应
- 人, 大鼠
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交叉反应 （详细）
- Probably won't recognize mouse.
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预测反应
- Rat - 11,6 amino acids residues identical
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产品特性
- Anti-MC1 Receptor Antibody is directed against an epitope of the human melanocortin receptor 1. Anti-MC1 Receptor Antibody (ABIN7043321, ABIN7044592 and ABIN7044593) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize MC1R from human and rat samples. The antibody is unlikely to recognize MC1R from mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R
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亚型
- IgG
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anti-Melanocortin 1 Receptor (MC1R) (AA 1-37) antibody
MC1R 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 1 Receptor (MC1R) (AA 271-317) antibodyMC1R 适用: 人, 小鼠 ELISA, WB, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 1 Receptor (MC1R) (C-Term) antibodyMC1R 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 1 Receptor (MC1R) (C-Term) antibodyMC1R 适用: 人 WB, IF, IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 1 Receptor (MC1R) (Cytoplasmic Domain) antibodyMC1R 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 1 Receptor (MC1R) antibodyMC1R 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Melanocortin 1 Receptor (MC1R) (AA 271-317) antibody (AbBy Fluor® 488)MC1R 适用: 人, 小鼠 WB, IF (cc), IF (p), FACS 宿主: 兔 Polyclonal AbBy Fluor® 488
anti-Melanocortin 1 Receptor (MC1R) (C-Term) antibodyMC1R 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 1 Receptor (MC1R) (AA 268-317) antibodyMC1R 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:400-1:500
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说明
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Cited Application: IHC|ICC
Negative Control: (ABIN7235916)
Blocking Peptide: (ABIN7235916)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- MC1 Receptor (MC1R) (Melanocortin 1 Receptor (MC1R))
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别名
- MC1R
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背景
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Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2,Melanocortin receptor 1 (MC1R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ-melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.MC1R was the first member of the melanocortin receptor family to be cloned. The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP. MC1R binds its ligands with the following potency: α-MSH = ACTH > β-MSH > γ-MSH. MC1R also binds the endogenous antagonist agouti with high affinity.1, 2MC1R can be described as the "classical" melanocyte α-MSH receptor. The receptor is expressed in the skin where it has a key role in determining skin and hair pigmentation. In fur-bearing mammals, the local ratio between α-MSH and agouti will determine coat pigmentation as α-MSH stimulates and agouti inhibits melanin production.In humans, especially in Caucasians, the MC1R gene is highly polymorphic and several allelic variants have been correlated with red-hair, poor tanning ability and increased risk of melanoma.1,3,5 In addition, MC1R expression in melanoma has been shown to be upregulated up to 20-fold when compared to normal melanocytes.The melanocortins and particularly α-MSH have significant anti-inflammatory properties. Since α-MSH binds to MC1R with the highest potency, it was proposed that the latter mediated the anti-inflammatory effects of α-MSH. Indeed, MC1R expression has been demonstrated in several cells of the immune system including macrophages and neutrophils.6
Alternative names: MC1 Receptor, Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2 -
基因ID
- 4157
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NCBI登录号
- NM_002386
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UniProt
- Q01726
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途径
- cAMP Metabolic Process, Feeding Behaviour
抗原
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