Leukotriene B4 Receptor/BLT 抗体 (Extracellular) (Atto 488)
Quick Overview for Leukotriene B4 Receptor/BLT 抗体 (Extracellular) (Atto 488) (ABIN7043308)
抗原
See all Leukotriene B4 Receptor/BLT (LTB4R) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 168-181, Extracellular
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原理
- A Rabbit Polyclonal Antibody to BLT1 Conjugated to the Fluorescent Dye ATTO-488
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特异性
- 2nd extracellular loop
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交叉反应 （详细）
- Not recommended for use with rat and mouse samples.
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预测反应
- Monkey - identical
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产品特性
- Anti-Human BLT1 (extracellular) Antibody (ABIN7043307 and ABIN7044557) is a highly specific antibody directed against an extracellular epitope of the human Leukotriene B4 receptor 1. The antibody can be used for western blot and indirect flow cytometry applications. It has been designed to recognize LTB4R from human samples only. \nAnti-Human BLT1 (extracellular)-ATTO Fluor-488 Antibody (ABIN7043308) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Human BLT1 (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human LTB4R
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亚型
- IgG
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anti-Leukotriene B4 Receptor (LTB4R) (AA 101-200) antibody
LTB4R 适用: 人, 小鼠, 大鼠 FACS, IHC (fro), IHC (p), IF (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) (Cytoplasmic Domain) antibodyLTB4R 适用: 人, 小鼠, 大鼠, 猴, Hamster, 马, 兔 IHC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) (C-Term) antibodyLTB4R 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) antibodyLTB4R 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) (AA 168-181), (Extracellular) antibodyLTB4R 适用: 人 FACS, WB 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) antibodyLTB4R 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) (AA 250-299) antibodyLTB4R 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582041)
Blocking Peptide:
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))
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别名
- LTB4R
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背景
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Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7,Leukotrienes (LTs) are lipid mediators prominently exerting proinflammatory responses. They are divided in two classes: cysteinyl LTs (cysLTs) which have a thioester linkage and LTB41. LTB4, a chemoattractant, plays a prominent role in the recruitment and activation of leukocytes2.LTB4 acts through two receptors which belong to the G-protein coupled receptor superfamily: BLT1 and BLT2. BLT1 displays high affinity for LTB4 and is highly expressed in leukocytes including granulocytes, macrophages, mast cells, dendritic cells and effector T cells3 and is expressed to much lower levels in the spleen, thymus, heart, skeletal muscle brain and liver2-4. BLT2 on the other hand is a low affinity receptor which does not display selectivity towards LTB45. Its expression is much more ubiquitous than that of BLT1. It is expressed in most human tissues, with the highest expression being in the spleen, liver, ovary, and leukocytes2,5-8. The two receptors could couple and activate different G-proteins, depending on the cell type and the cellular events activating the receptors2. However, activation of BLT1 usually correlates with increasing intracellular concentrations of Ca2+.The LTB4/BLT1 system is involved in many allergic reactions as well as asthma induced as an allergic response, where it plays a significant role in recruiting neutrophils and effector T cells into lungs as part of an inflammatory response induced by allergens1.
Alternative names: BLT1, Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7 -
基因ID
- 1241
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NCBI登录号
- NM_001143919
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UniProt
- Q15722
抗原
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