Latrophilin 1 抗体 (Extracellular)
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Quick Overview for Latrophilin 1 抗体 (Extracellular) (ABIN7043297)
抗原
See all Latrophilin 1 (LPHN1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 480-494, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Latrophilin-1 Receptor
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,human - identical
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产品特性
- Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (ABIN7043297 and ABIN7044563) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Latrophilin-1 from rat, human, and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CEPREVRRVQWPATQ(G), corresponding to amino acid residues 480-494 of rat Latrophilin-1
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亚型
- IgG
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anti-Latrophilin 1 (LPHN1) (N-Term) antibody
LPHN1 适用: 大鼠, 人, 小鼠, Cow, 犬, 猴, Hamster, Bat, Pig, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 1 (LPHN1) (C-Term) antibodyLPHN1 适用: 大鼠, 人, 小鼠, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 1 (LPHN1) (AA 1-201) antibodyLPHN1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 1 (LPHN1) (AA 1-201) antibodyLPHN1 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 4E12 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235844)
Blocking Peptide: (ABIN7235844)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Latrophilin 1 (LPHN1)
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别名
- ADGRL1
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背景
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Calcium-independent alpha-latrotoxin receptor 1, CIRL-1, Adhesion G protein-coupled receptor L1, ADGRL1, Lectomedin-2, LEC2,Latrophilins (Latrophilin1-3) are members of the adhesion G-protein coupled receptor subfamily. Like all GPCRs, Latrophilins have seven transmembrane domains and are distinguished by a large extracellular N-terminal tail and a large intracellular C-terminal tail1. The N-terminus has several cell adhesion domains and undergoes proteolysis after synthesis, while the C-terminal has various consensus post-translational sites like phosphorylation and palmitoylation2. In addition, Latrophilins undergo alternative splicing3.Latrophilin-1 was discovered by its ability to bind α-Latrotoxin (α-LTX), a toxin isolated from the black widow spider venom4. α-LTX induces exocytosis by creating a Ca2+ influx in the presynaptic membrane. α-LTX can also stimulate small vesicle exocytosis in a Ca2+ independent manner. Three receptors have been found to bind α-LTX. Of the three, Latrophilins are responsible for the Ca2+-independent effects of α-LTX2. The binding of α-LTX to Latrophilin-1 increases exocytosis of neurotransmitters5,6.In an attempt to find the natural ligand of Latrophilin-1, Lasso, a splice variant of Teneneurin-2 was discovered to be an endogenous binding partner of the adhesion-GPCR7. Teneneurins are large glycoproteins with a single transmembrane domain8,9. Like Latrophilin-1, Teneneurins are mostly expressed in the brain where they modulate neurite outgrowth, axon guidance and synaptogenesis7.Regarding the localization of Latrophilins, Latrophilin-1 is expressed predominantly in the brain, Latrophilin-2 is highly expressed in the liver and lung, while Latrophilin-3 is almost exclusively detected in the brain1,10.
Alternative names: Latrophilin-1 (LPHN1), Calcium-independent alpha-latrotoxin receptor 1, CIRL-1, Adhesion G protein-coupled receptor L1, ADGRL1, Lectomedin-2, LEC2 -
基因ID
- 65096
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NCBI登录号
- NM_014921
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UniProt
- O88917
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途径
- Synaptic Membrane, Synaptic Vesicle Exocytosis
抗原
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