HCRTR1 抗体 (AA 256-269)
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- 抗原 See all HCRTR1 抗体
- HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))
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抗原表位
- AA 256-269
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HCRTR1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)
- 原理
- A Rabbit Polyclonal Antibody to Orexin Receptor 1
- 特异性
- 3rd intracellular loop
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Mouse - identical, human - 9,14 amino acid residues identical
- 产品特性
- Anti-Orexin Receptor 1 Antibody (ABIN7043285, ABIN7044883 and ABIN7044884) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX1R from rat, mouse, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R
- 亚型
- IgG
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anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) antibody
HCRTR1 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) (Extracellular Domain) antibodyHCRTR1 适用: 人, 大鼠, 兔, Cow, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) (C-Term) antibodyHCRTR1 适用: 人, 犬, 马, 猴, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) (Cytoplasmic Domain) antibodyHCRTR1 适用: 人, 大鼠, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) (AA 150-250) antibodyHCRTR1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) (AA 101-200) antibodyHCRTR1 适用: 人 ELISA, WB, IHC (p), IF (cc), IF (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) (AA 1-46) antibodyHCRTR1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) (AA 256-291) antibodyHCRTR1 适用: 人 WB 宿主: 兔 Polyclonal RB51454 unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) antibodyHCRTR1 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Hypocretin (Orexin) Receptor 1 (HCRTR1) (C-Term) antibodyHCRTR1 适用: 人, 大鼠, 小鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50-1:100
Application Dilutions Western blot wb: 1:200-1:500
- 说明
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Cited Application: IHC
Negative Control: (ABIN7235802)
Blocking Peptide: (ABIN7235802)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))
- 别名
- HCRTR1 (HCRTR1 产品)
- 背景
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OX1R, Hypocretin receptor type 1, HCRTR1,Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4
Alternative names: Orexin Receptor 1, OX1R, Hypocretin receptor type 1, HCRTR1 - 基因ID
- 25593
- NCBI登录号
- NM_001525
- UniProt
- P56718
- 途径
- Feeding Behaviour
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