HRH2 抗体 (Extracellular)
Quick Overview for HRH2 抗体 (Extracellular) (ABIN7043270)
抗原
See all HRH2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 161-173, Extracellular
-
原理
- A Rabbit Polyclonal Antibody to Histamine H2 Receptor
-
特异性
- 2nd extracellular loop
-
交叉反应
- 小鼠, 大鼠
-
交叉反应 （详细）
- Will not recognize human histamine H2 receptor.
-
预测反应
- Mouse - identical
-
产品特性
- Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody is directed against an epitope of rat histamine H2 receptor. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (ABIN7043270, ABIN7044507 and ABIN7044508) can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize H2R from rat and mouse samples. It will not recognize human H2R.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat HRH2
-
亚型
- IgG
-
-
anti-Histamine Receptor H2 (HRH2) (C-Term) antibody
Verified HRH2 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Histamine Receptor H2 (HRH2) (N-Term) antibodyHRH2 适用: 人 WB, IF, IHC (p), IHC, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H2 (HRH2) (AA 161-259) antibodyHRH2 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H2 (HRH2) (Cytoplasmic Domain) antibodyHRH2 适用: 人, 猴 IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H2 (HRH2) (AA 309-323) antibodyVerified HRH2 适用: 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Histamine Receptor H2 (HRH2) (Extracellular Domain) antibodyHRH2 适用: 人, 大鼠 IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H2 (HRH2) (Internal Region) antibodyHRH2 适用: 人, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H2 (HRH2) (AA 150-250) antibodyHRH2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H2 (HRH2) (Center) antibodyHRH2 适用: 人, 大鼠, 小鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
-
说明
-
Cited Application: IHC
Negative Control: (ABIN7235754)
Blocking Peptide: (ABIN7235754)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- 0.2 mL double distilled water (DDW).
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- HRH2 (Histamine Receptor H2 (HRH2))
-
别名
- HRH2
-
背景
-
H2R, HH2R, Gastric receptor I, Gastric receptor 1,Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2 H2 receptors are expressed in several tissues including the brain, gastrointestinal tract and heart.The most studied physiological role of the H2 receptor is its involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia.Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested.
Alternative names: Histamine H2 Receptor (HRH2), H2R, HH2R, Gastric receptor I, Gastric receptor 1 -
基因ID
- 25461
-
NCBI登录号
- NM_001131055
-
UniProt
- P25102
抗原
-
