GRM8 抗体 (Extracellular)
Quick Overview for GRM8 抗体 (Extracellular) (ABIN7043256)
抗原
See all GRM8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 365-377, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 8
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,mouse,human - 12,13 amino acid residues identical
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产品特性
- Anti-mGluR8 (extracellular) Antibody is directed against an extracellular epitope of rat mGluR8. Anti-mGluR8 (extracellular) Antibody (ABIN7043256 and ABIN7044363) can be used in western blot and immunohistochemistry applications. It has been designed to recognize mGluR8 from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365-377 of rat mGluR8
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亚型
- IgG
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anti-Glutamate Receptor, Metabotropic 8 (GRM8) (AA 440-583) antibody
GRM8 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4C1C1 unconjugated
anti-Glutamate Receptor, Metabotropic 8 (GRM8) antibodyGRM8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 8 (GRM8) (N-Term) antibodyGRM8 适用: 人, 小鼠, 大鼠, 猴, 兔, Bat, 马, 小鸡, 犬, Hamster, 绵羊 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 8 (GRM8) (C-Term) antibodyGRM8 适用: 人, 小鼠, 大鼠, 猴, Bat, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 8 (GRM8) (AA 486-575) antibodyGRM8 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A7 unconjugated
anti-Glutamate Receptor, Metabotropic 8 (GRM8) (AA 486-575) antibodyGRM8 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 8 (GRM8) (AA 841-890) antibodyGRM8 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Metabotropic 8 (GRM8) (Extracellular Domain), (N-Term) antibodyGRM8 适用: 人 IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:400
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说明
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Negative Control: (ABIN7235709)
Blocking Peptide: (ABIN7235709)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))
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别名
- GRM8
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背景
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Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H,Metabotropic glutamate receptors (mGluRs), heptahelical transmembrane receptors coupled to G-proteins, contribute to the regulation of neuronal excitability and synaptic transmission1. Genes coding for eight mGluRs (denoted mGluR1-8) with different splice variants have been identified and classified into three groups based on sequence homology, pharmacology, and signaling pathways. Group I mGluRs (mGluR1 and mGluR5) are primarily located postsynaptically. Group II (mGluR2 and mGluR3) are present both postsynaptically and presynaptically outside the release site. Group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) couple to Gαi/o to inhibit adenylyl cyclase found in the presynaptic active zone2.The mGluR8 receptor is detected in the olfactory bulb, thalamus, pontine gray, cerebral cortex, hippocampus, cerebellum, and retina3. Electrophysiological studies suggest that mGluR8 functions as a presynaptic autoreceptor to regulate glutamate release from the lateral perforant path terminals in the mouse dentate gyrus4. It is suggested that mGluR8, like other group III mGluRs, controls glutamate release by inhibiting voltage-gated Ca2+ channels.Targeted deletion of the mGluR8 gene in mice produces subtle behavioral alterations such as reduced habituation to novelty, hyperactivity, context-dependent but not cue-dependent disruption of the freezing response in the fear conditioning test, and increased anxiety5.
Alternative names: mGluR8, Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H -
基因ID
- 60590
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NCBI登录号
- NM_000845
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UniProt
- P70579
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途径
- cAMP Metabolic Process, Synaptic Membrane
抗原
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