GRIN1/NMDAR1 抗体 (Extracellular)
Quick Overview for GRIN1/NMDAR1 抗体 (Extracellular) (ABIN7043243)
抗原
See all GRIN1/NMDAR1 (GRIN1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Extracellular
-
原理
- A Guinea Pig Polyclonal Antibody to NMDA Receptor 1 (GluN1)
-
特异性
- Extracellular, N-terminus
-
交叉反应
- 人, 小鼠, 大鼠
-
预测反应
- Human,mouse - identical
-
产品特性
- Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody is directed against an extracellular epitope of rat NMDA Receptor 1. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody raised in guinea pig can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting NMDAR1 in living cells. It has been designed to recognize NMDAR1 from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-NMDAR1 (GluN1) (extracellular) Antibody (ABIN7043242, ABIN7044322 and ABIN7044323) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: ETEKPRGYQMSTRLK(C), corresponding to amino acid residues 385-399 of rat GluN1
-
亚型
- IgG
-
-
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (Internal Region) antibody
Verified GRIN1 适用: 人, 小鼠 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 600-700) antibodyGRIN1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (C-Term) antibodyGRIN1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (pSer890) antibodyGRIN1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 1-564) antibodyGRIN1 适用: 大鼠 WB, IP 宿主: 小鼠 Monoclonal R1JHL unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 42-361) antibodyGRIN1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S308-48 unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 42-361) antibody (HRP)GRIN1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S308-48 HRP
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 42-361) antibody (FITC)GRIN1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S308-48 FITC
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 42-361) antibody (Biotin)GRIN1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S308-48 Biotin
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 42-361) antibody (PE)GRIN1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S308-48 PE
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600-1:1200
Application Dilutions Western blot wb: 1:200
-
说明
-
Negative Control: (ABIN7235673)
Blocking Peptide: (ABIN7235673)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))
-
别名
- GRIN1
-
背景
-
NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1,The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.
Alternative names: NMDAR1 (GluN1), NMDA receptor 1, Ionotropic glutamate receptor subunit zeta1, N-methyl-D-aspartate receptor subunit 1, GRIN1, GLUN1 -
基因ID
- 24408
-
NCBI登录号
- NM_007327
-
UniProt
- P35439
-
途径
- Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
抗原
-
